PMID- 8697421
OWN - NLM
STAT- MEDLINE
DCOM- 19960830
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 89
IP  - 2
DP  - 1996 Jul 15
TI  - Restriction endonuclease in situ digestion (REISD) and fluorescence in situ 
      hybridization (FISH) as complementary methods to analyze chimerism and residual 
      disease after bone marrow transplantation.
PG  - 141-5
AB  - The efficiency of restriction endonuclease in situ digestion (REISD) with Sau3A 
      to analyze chimerism and residual disease (RD) has been tested before and after 
      an allogenic bone marrow transplant (BMT) in an acute lymphoblastic leukemia 
      (ALL) patient. The combined results obtained with REISD and FISH using the 
      appropriate probes for detecting chromosome rearrangements have proven to be 
      useful for the identification and quantification of both the hemopoietic 
      chimerism achieved after BMT and the RD persistent in the patient. The 
      sensitivity of REISD has been determined to be around 95%, i.e., similar to that 
      obtained by FISH. REISD with Sau3A was particularly useful in the analysis of 
      chimerism since this enzyme revealed the polymorphic status of constitutive 
      heterochromatin in human chromosome 3 and thus allowed discrimination of cells 
      derived from donor and recipient. The method itself seems promising since neither 
      a donor/recipient sex mismatch nor a cytogenetic disease marker are needed for 
      its application.
FAU - Gosalvez, J
AU  - Gosalvez J
AD  - Unidad de Genetica, Universidad Autonoma de Madrid, Spain.
FAU - Lopez-Fernandez, C
AU  - Lopez-Fernandez C
FAU - Buno, I
AU  - Buno I
FAU - Polo, N
AU  - Polo N
FAU - Llamas, P
AU  - Llamas P
FAU - Fernandez, M N
AU  - Fernandez MN
FAU - Fernandez, J L
AU  - Fernandez JL
FAU - Diez-Martin, J L
AU  - Diez-Martin JL
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GATC-specific type II deoxyribonucleases)
SB  - IM
MH  - Adult
MH  - *Bone Marrow Transplantation
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 9
MH  - DNA Restriction Enzymes/*metabolism
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/*therapy
MH  - *Transplantation Chimera
EDAT- 1996/07/15 00:00
MHDA- 1996/07/15 00:01
CRDT- 1996/07/15 00:00
PHST- 1996/07/15 00:00 [pubmed]
PHST- 1996/07/15 00:01 [medline]
PHST- 1996/07/15 00:00 [entrez]
AID - 0165-4608(95)00181-6 [pii]
AID - 10.1016/0165-4608(95)00181-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Jul 15;89(2):141-5. doi: 
      10.1016/0165-4608(95)00181-6.