PMID- 8697434
OWN - NLM
STAT- MEDLINE
DCOM- 19960830
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 89
IP  - 2
DP  - 1996 Jul 15
TI  - Detection of chromosomal abnormalities in uterine leiomyoma using conventional 
      cytogenetic method and interphase fluorescence in situ hybridization.
PG  - 98-104
AB  - Seventy-nine uterine leiomyomas were examined using a conventional cytogenetic 
      method and fluorescence in situ hybridization (FISH) for detection of chromosomal 
      abnormalities of chromosome 12. Nine (17.6%) of 51 tumor samples examined showed 
      chromosomal abnormalities by conventional cytogenetic analysis. Rearrangements of 
      chromosome 12 were detected in two tumors. Other tumors showed abnormalities 
      affecting chromosomes 1, 4, 6, 7, 10, 13, 14, and 22. For FISH, the 
      whole-chromosome painting probe and the D12Z3 probe specific for the centromeric 
      region were used to detect structural and numerical abnormalities of chromosome 
      12. Of forty-one tumor samples, six showed structural aberrations and four showed 
      numerical aberrations of chromosome 12 by FISH analysis. Of the tumors with 
      structural aberrations identified by FISH, two had normal karyotypes, two showed 
      structural rearrangements of chromosome 12 cytogenetically, and two could not be 
      analyzed because of an insufficient number of metaphases. There were no 
      correlations between the cytogenetic data and clinical parameters. The results 
      indicate that chromosomal abnormalities are important in the biology of at least 
      some types of uterine leiomyomas, and that FISH is a useful complement to 
      conventional cytogenetic analysis in the study of solid tumors.
FAU - Hayashi, S
AU  - Hayashi S
AD  - Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, 
      Japan.
FAU - Miharu, N
AU  - Miharu N
FAU - Okamoto, E
AU  - Okamoto E
FAU - Samura, O
AU  - Samura O
FAU - Hara, T
AU  - Hara T
FAU - Ohama, K
AU  - Ohama K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 12
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Leiomyoma/*genetics
MH  - Leiomyomatosis/genetics
MH  - Middle Aged
MH  - Uterine Neoplasms/*genetics
EDAT- 1996/07/15 00:00
MHDA- 1996/07/15 00:01
CRDT- 1996/07/15 00:00
PHST- 1996/07/15 00:00 [pubmed]
PHST- 1996/07/15 00:01 [medline]
PHST- 1996/07/15 00:00 [entrez]
AID - 0165-4608(95)00354-1 [pii]
AID - 10.1016/0165-4608(95)00354-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1996 Jul 15;89(2):98-104. doi: 
      10.1016/0165-4608(95)00354-1.