PMID- 8697584
OWN - NLM
STAT- MEDLINE
DCOM- 19960905
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 42
IP  - 8 Pt 1
DP  - 1996 Aug
TI  - Evaluation of a two-site immunoradiometric assay for measuring noncomplexed 
      (free) prostate-specific antigen.
PG  - 1243-9
AB  - Serum prostate-specific antigen (PSA) in men is present as two different 
      molecular forms separable by gel-filtration chromatography (GFC). We have 
      evaluated a two-site IRMA that measures only the noncomplexed (free) form of PSA 
      (F-PSA). Verification that the F-PSA assay measures solely F-PSA was obtained by 
      assaying GFC-fractionated serum samples with both the F-PSA IRMA and a commercial 
      PSA assay that measures total PSA (T-PSA: F-PSA plus alpha 
      1-antichymotrypsin-complexed PSA). The F-PSA assay detected only the 30-kDa peak 
      corresponding to the free form of PSA, whereas the T-PSA assay detected two 
      peaks: complexed PSA at approximately 90 kDa and F-PSA at approximately 30 kDa. 
      The F-PSA assay had an analytical detection limit of 0.03 microgram/L and a 
      measuring range up to 50 micrograms/L. The intraassay CV was 1.7-10% in the 
      concentration range of 0.2-30 micrograms/L. The interassay CV was 3.4-12.5% in 
      the same concentration range. Dilution and recovery studies showed no significant 
      deviation from linearity across the assay range. The assay was insensitive to 
      interference from hemoglobin, bilirubin, and total lipids up to concentrations of 
      5, 0.2, and 10 g/L, respectively. No significant loss of immunological activity 
      (analyte stability) was seen day-to-day ( < or = 5) or after repeated freeze/thaw 
      ( < or = 5) cycles. We conclude that the F-PSA IRMA is an accurate, precise, and 
      reliable tool for measuring F-PSA in human serum.
FAU - Cuny, C
AU  - Cuny C
AD  - DIANON Systems, Inc., Stratford, CT 06497, USA.
FAU - Pham, L
AU  - Pham L
FAU - Kramp, W
AU  - Kramp W
FAU - Sharp, T
AU  - Sharp T
FAU - Soriano, T F
AU  - Soriano TF
LA  - eng
PT  - Journal Article
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (alpha 1-Antichymotrypsin)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
SB  - IM
MH  - Aged
MH  - Antibodies, Monoclonal
MH  - Binding, Competitive
MH  - Chromatography, Gel
MH  - Drug Stability
MH  - Humans
MH  - Immunoradiometric Assay/*methods/statistics & numerical data
MH  - Male
MH  - Middle Aged
MH  - Prostate-Specific Antigen/*blood
MH  - Prostatic Neoplasms/blood
MH  - Protein Binding
MH  - Sensitivity and Specificity
MH  - alpha 1-Antichymotrypsin/blood
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1996 Aug;42(8 Pt 1):1243-9.