PMID- 8697814 OWN - NLM STAT- MEDLINE DCOM- 19960903 LR - 20180214 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 73 IP - 3 DP - 1996 TI - T-banding pattern of bovine chromosomes and karyotype reconstitution with physically mapped cosmids. PG - 229-34 AB - Bovine T-banded chromosomes were identified by fluorescence in situ hybridization (FISH) using 27 cosmid probes as chromosome landmarks. Pairwise combinations of probes from chromosomes of markedly different size were cohybridized to metaphase spreads of T-banded chromosomes. This confirmed the association of banding patterns to individual chromosomes. The T-banding pattern appears to be related to R-bands: 80% of T-bands were in corresponding position to R-bands, but not always conserved in size. Band assignments placed 20 probes on similar bands in both patterns. The identification of T-banded chromosomes represents a first step toward the construction of a compositional map of the bovine genome and an additional tool for its study on a comparative basis. FAU - Mezzelani, A AU - Mezzelani A AD - DVGA, National Council of Research (CNR), Milano, Italy. FAU - Castiglioni, B AU - Castiglioni B FAU - Eggen, A AU - Eggen A FAU - Ferretti, L AU - Ferretti L LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 SB - IM MH - Animals MH - Cattle/*genetics MH - Cells, Cultured MH - Chromosome Banding/*veterinary MH - Chromosome Mapping/veterinary MH - Cosmids MH - Karyotyping/veterinary EDAT- 1996/01/01 00:00 MHDA- 1996/01/01 00:01 CRDT- 1996/01/01 00:00 PHST- 1996/01/01 00:00 [pubmed] PHST- 1996/01/01 00:01 [medline] PHST- 1996/01/01 00:00 [entrez] AID - 10.1159/000134345 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1996;73(3):229-34. doi: 10.1159/000134345.