PMID- 8698398
OWN - NLM
STAT- MEDLINE
DCOM- 19960905
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 87
IP  - 2
DP  - 1996 Feb
TI  - Stem cell factor enhances immunoglobulin E-dependent mediator release from 
      cultured rat bone marrow-derived mast cells: activation of previously 
      unresponsive cells demonstrated by a novel ELISPOT assay.
PG  - 326-33
AB  - Mucosal mast cells (MMC) are important effector cells in the immune response 
      against gastrointestinal nematodes. We used cultured rat bone marrow-derived mast 
      cells (BMMC) as an in vitro model of MMC to study the effects of the 
      multifunctional cytokine stem cell factor (SCF) on immunoglobulin E 
      (IgE)-dependent secretion of granule mediators. SCF (< or = 1000 ng/ml) was not a 
      direct secretagogue for these cells, but it significantly enhanced IgE-mediated 
      secretion of the granule constituents rat mast cell protease-II (RMCP-II) and 
      beta-hexosaminidase from mature BMMC in a dose-dependent manner (> 10 ng/ml). 
      Maximum up-regulation of secretion occurred after cells were pretreated with SCF 
      (50 ng/ml) for 5 minutes before challenge with anti-IgE, but the effect then 
      declined and was absent in cells incubated with the cytokine for 3 to 24 h. In a 
      novel ELISPOT assay developed to identify individual BMMC secreting RMCP-II, the 
      proportion of mature BMMC responding to anti-IgE was significantly increased by 
      treatment with SCF. To investigate this effect further, the percentage release of 
      RMCP-II and beta-hexosaminidase from populations of mature BMMC was directly 
      compared to the proportion of individual cells releasing RMCP-II as detected by 
      ELISPOT. The release of both mediators was enhanced by SCF, and the increased 
      percentage release reflected both an increased proportion of secreting cells, and 
      enhanced mediator release from individual cells. These results suggest that SCF 
      can enhance IgE-dependent mediator release from BMMC not only by augmenting the 
      secretory response from individual cells, but also by activating previously 
      unresponsive cells.
FAU - Hill, P B
AU  - Hill PB
AD  - Department of Preclinical Veterinary Sciences, University of Edinburgh, UK.
FAU - MacDonald, A J
AU  - MacDonald AJ
FAU - Thornton, E M
AU  - Thornton EM
FAU - Newlands, G F
AU  - Newlands GF
FAU - Galli, S J
AU  - Galli SJ
FAU - Miller, H R
AU  - Miller HR
LA  - eng
GR  - AI 22674/AI/NIAID NIH HHS/United States
GR  - AI 23990/AI/NIAID NIH HHS/United States
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Stem Cell Factor)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (chymase 2)
RN  - EC 3.4.21.- (cytotoxic T lymphocyte-specific serine protease)
RN  - EC 3.4.21.39 (Chymases)
SB  - IM
MH  - Animals
MH  - Bone Marrow/immunology/*metabolism
MH  - Cell Culture Techniques
MH  - Chymases
MH  - Dose-Response Relationship, Immunologic
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin E/*physiology
MH  - Kinetics
MH  - Male
MH  - Mast Cells/immunology/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Serine Endopeptidases/*metabolism
MH  - Stem Cell Factor/*pharmacology
MH  - beta-N-Acetylhexosaminidases/*metabolism
PMC - PMC1384292
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
PMCR- 1997/02/01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PHST- 1997/02/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1996.455545.x [doi]
PST - ppublish
SO  - Immunology. 1996 Feb;87(2):326-33. doi: 10.1046/j.1365-2567.1996.455545.x.