PMID- 8699073
OWN - NLM
STAT- MEDLINE
DCOM- 19960905
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 174
IP  - 2
DP  - 1996 Aug
TI  - Measurement of human herpesvirus 7 load in peripheral blood and saliva of healthy 
      subjects by quantitative polymerase chain reaction.
PG  - 396-401
AB  - Qualitative and competitive-quantitative nested polymerase chain reaction (PCR) 
      assays were developed for human herpesvirus 7 (HHV-7). These assays amplify a DNA 
      sequence encoding part of the HHV-7 homologue of the human herpesvirus 6 (HHV-6) 
      U42 gene. The PCR assays were used to analyze peripheral blood DNA (pbDNA) and 
      saliva from 24 healthy volunteers. The prevalence of HHV-7 in saliva was 96%, 
      with a median virus load of 1.1 x 10(6) copies/mL. Longitudinal analysis revealed 
      sustained virus load, suggesting continued active viral replication. Analysis of 
      1 microgram of pbDNA showed the prevalence of HHV-7 to be 83%, with a median 
      virus load of 40 copies (267 copies/10(6) cells). Analysis of sequential pbDNA 
      samples showed individuals to have stable levels of HHV-7 virus load. These data 
      demonstrate persistence of HHV-7 at two distinct sites and provide baseline data 
      allowing comparisons with HHV-7 load in immunocompromised patients.
FAU - Kidd, I M
AU  - Kidd IM
AD  - Department of Virology, Royal Free Hospital School of Medicine, Hampstead, 
      London, United Kingdom.
FAU - Clark, D A
AU  - Clark DA
FAU - Ait-Khaled, M
AU  - Ait-Khaled M
FAU - Griffiths, P D
AU  - Griffiths PD
FAU - Emery, V C
AU  - Emery VC
LA  - eng
GR  - AI-33389/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (DNA Primers)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Genes, Viral
MH  - Herpesviridae Infections/blood/*virology
MH  - Herpesvirus 6, Human/genetics
MH  - Herpesvirus 7, Human/genetics/*isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - Regulatory Sequences, Nucleic Acid
MH  - Saliva/*virology
MH  - Time Factors
MH  - Viral Proteins/genetics
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1093/infdis/174.2.396 [doi]
PST - ppublish
SO  - J Infect Dis. 1996 Aug;174(2):396-401. doi: 10.1093/infdis/174.2.396.