PMID- 8699933
OWN - NLM
STAT- MEDLINE
DCOM- 19960830
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 59
IP  - 3
DP  - 1996
TI  - Effect of growth hormone, insulin and dexamethasone on 11 beta-hydroxysteroid 
      dehydrogenase activity on a primary culture of rat hepatocytes.
PG  - 227-34
AB  - 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) is a microsomal enzyme present 
      in the peripheral tissues of the rat, including the liver, and is mediated by a 
      number of factors in animal in vivo studies. However, the effect of peptide 
      hormones and glucocorticoids on the activity of 11 beta-HSD in isolated rat 
      hepatocytes is not clear. To investigate these effects, we determined 11 beta-HSD 
      activity in a primary culture of rat hepatocytes by adding various concentrations 
      of growth hormone, insulin and dexamethasone (Dex). 11 beta-HSD activity 
      increased significantly after treatment with Dex (10(-9)M-10(-6)M) for 48h. Dex 
      (100nM) treated hepatocytes, incubated for 12h to 48h, resulted in a significant 
      two-to four-fold rise in 11 beta-HSD activity compared to control (p < 0.01), 
      which was in contrast to GH (10(-9)M-10(-6)M) and insulin (10(-8)M-10(-5)M), 
      which inhibited 11 beta-HSD activity (p < 0.05). These results suggest that the 
      11 beta-HSD of rat hepatocytes is under multifactorial regulation; Dex stimulates 
      and GH and insulin inhibit 11 beta-HSD activity in primary cultures of rat 
      hepatocytes.
FAU - Liu, Y J
AU  - Liu YJ
AD  - Department of Pediatrics, Hamamatsu University School of Medicine, Japan.
FAU - Nakagawa, Y
AU  - Nakagawa Y
FAU - Nasuda, K
AU  - Nasuda K
FAU - Saegusa, H
AU  - Saegusa H
FAU - Igarashi, Y
AU  - Igarashi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Insulin)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9002-72-6 (Growth Hormone)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Cells, Cultured
MH  - Dexamethasone/*pharmacology
MH  - Growth Hormone/*pharmacology
MH  - Hydroxysteroid Dehydrogenases/*pharmacology
MH  - Insulin/*pharmacology
MH  - Liver/*drug effects
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 0024320596002883 [pii]
AID - 10.1016/0024-3205(96)00288-3 [doi]
PST - ppublish
SO  - Life Sci. 1996;59(3):227-34. doi: 10.1016/0024-3205(96)00288-3.