PMID- 8700182
OWN - NLM
STAT- MEDLINE
DCOM- 19960904
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 369
IP  - 1-2
DP  - 1996 Jul 10
TI  - Detection of neocarzinostatin-induced translocations in human sperm chromosomes 
      using fluorescence in situ hybridization of chromosome 2.
PG  - 51-8
AB  - Mature sperm and late spermatid are known to be sensitive stages to clastogens in 
      mammalian spermatogenesis. Certain types of chromosomal damage induced in these 
      stages will pass to successive generations as heritable translocations. In the 
      present study, we employed whole chromosome 2 painting with the fluorescence in 
      situ hybridization (FISH) technique to detect the chemically induced 
      translocations in human sperm. Mature human sperm were treated in vitro with an 
      antitumor drug, neocarzinostatin (NCS), and fertilized in vitro with golden 
      hamster oocytes. Sperm pronuclear chromosome slides were prepared at the first 
      cleavage metaphase. To compare the characteristics of translocations between 
      somatic and germ cells, human lymphocytes in peripheral blood treated with NCS in 
      vitro were analyzed at first round metaphase after PHA-stimulation. From the 
      analysis of translocations by whole chromosome 2 painting, frequencies of the 
      haploid genomic translocations (FhG) were predicted for both sperm and 
      lymphocytes. At 1.0 micrograms/ml, the actual percentages of sperm and 
      lymphocytes with chromosome 2 translocations were almost identical (11.9% and 
      12.0%). At the same dose, however, the FhG of the sperm (1.15) was considerably 
      higher than that of the lymphocytes (0.58), indicating that complex 
      translocations having two or more rearranged sites were induced by NCS more 
      frequently in sperm than in lymphocytes.
FAU - Kusakabe, H
AU  - Kusakabe H
AD  - Department of Cellular and Genetic Toxicology, Hatano Research Institute, 
      Kanagawa, Japan.
FAU - Yamakage, K
AU  - Yamakage K
FAU - Tanaka, N
AU  - Tanaka N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - 9014-02-2 (Zinostatin)
SB  - IM
MH  - Animals
MH  - *Chromosomes, Human, Pair 2
MH  - Cricetinae
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/drug effects
MH  - Male
MH  - Mesocricetus
MH  - Mutagens/*toxicity
MH  - Spermatozoa/*drug effects
MH  - *Translocation, Genetic
MH  - Zinostatin/*toxicity
EDAT- 1996/07/10 00:00
MHDA- 1996/07/10 00:01
CRDT- 1996/07/10 00:00
PHST- 1996/07/10 00:00 [pubmed]
PHST- 1996/07/10 00:01 [medline]
PHST- 1996/07/10 00:00 [entrez]
AID - S0165-1218(96)90047-6 [pii]
AID - 10.1016/s0165-1218(96)90047-6 [doi]
PST - ppublish
SO  - Mutat Res. 1996 Jul 10;369(1-2):51-8. doi: 10.1016/s0165-1218(96)90047-6.