PMID- 8700531
OWN - NLM
STAT- MEDLINE
DCOM- 19960904
LR  - 20211203
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 12
IP  - 12
DP  - 1996 Jun 20
TI  - Tissue-specific expression, evolutionary conservation and localization of the cph 
      proto-oncogene on Syrian hamster chromosome X.
PG  - 2713-7
AB  - Treatment of Syrian hamster embryo fibroblasts with a single dose of 
      3-methylcholanthrene caused the activation of the transforming potential of 
      cellular sequences (Notario et al, Oncogene 5: 1425-1430, 1990), which were 
      subsequently isolated by cosmid rescue techniques, and further identified as a 
      novel oncogene, termed cph because of its involvement in the carcinogenic 
      progression of hamster embryo cells (Velasco et al, Oncogene 9: 2065-2069, 1994). 
      We have analysed the expression of the cph proto-oncogene in adult Syrian hamster 
      tissues by northern hybridization using cph-specific genomic probes. The three 
      cph transcripts expressed in normal and neoplastic Syrian hamster embryo cells in 
      culture (5.0, 3.5 and 2.0 kb) were also present in most adult tissues, although 
      different mRNA species, most likely resulting from alternative splicing events, 
      were expressed in testes. The highest steady-state level of cph mRNA was found in 
      kidney, whereas cph expression was nearly undetectable in skin and skeletal 
      muscle. Southern blot analyses of DNAs from other eucaryotic organisms were 
      performed under moderate stringency conditions with a Syrian hamster-specific cph 
      probe. Discrete cph-hybridizing sequences were present in genomes from yeast to 
      mammalian species, including humans, thus demonstrating that cph is a highly 
      conserved gene in eucaryotic evolution. Using fluorescence in situ hybridization 
      (FISH), we have determined also the chromosomal localization of the cph 
      proto-oncogene in the hamster genome. FISH experiments demonstrated that cph is a 
      single copy gene, localized on the euchromatic short arm of the X chromosome, at 
      region Xpa7. Because chromosome X is frequently involved in structural 
      alterations in neoplastic Syrian hamster cells transformed by chemical 
      carcinogens and oncogenic viruses, the localization of the cph locus on this 
      chromosome supports the notion that the cph oncogene plays a role in the 
      malignant conversion of chemically transformed hamster fibroblasts. The wide 
      range of tissue-specific expression and species-specific distribution of cph 
      strongly suggest that the normal function of the cph protein product(s) may be 
      essential for metabolic processes involved in the regulation of cell 
      proliferation and survival.
FAU - Velasco, J A
AU  - Velasco JA
AD  - Department of Radiation Medicine, Georgetown University Medical Center, 
      Washington DC, USA.
FAU - Zimonjic, D B
AU  - Zimonjic DB
FAU - Popescu, N C
AU  - Popescu NC
FAU - Cansado, J
AU  - Cansado J
FAU - DiPaolo, J A
AU  - DiPaolo JA
FAU - Albor, A
AU  - Albor A
FAU - Notario, V
AU  - Notario V
LA  - eng
GR  - CA64472/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Conserved Sequence
MH  - Cricetinae
MH  - Embryo, Mammalian/cytology
MH  - *Evolution, Molecular
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Mesocricetus/embryology/*genetics/growth & development
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogenes/*genetics/physiology
MH  - Sequence Homology, Nucleic Acid
MH  - Testis/chemistry/metabolism
MH  - Tissue Distribution
MH  - Transcription, Genetic
MH  - *X Chromosome
EDAT- 1996/06/20 00:00
MHDA- 1996/06/20 00:01
CRDT- 1996/06/20 00:00
PHST- 1996/06/20 00:00 [pubmed]
PHST- 1996/06/20 00:01 [medline]
PHST- 1996/06/20 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1996 Jun 20;12(12):2713-7.