PMID- 8701989
OWN - NLM
STAT- MEDLINE
DCOM- 19960904
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 149
IP  - 2
DP  - 1996 Aug
TI  - Monocyte chemoattractant protein-1 gene expression in prostatic hyperplasia and 
      prostate adenocarcinoma.
PG  - 501-9
AB  - Human monocyte chemoattractant protein-1 (MCP-1) has been shown to act as a 
      chemokine in the recruitment of monocyte/macrophages during inflammation states. 
      Furthermore, there is increasing evidence that MCP-1 is involved in the 
      recruitment of tumor-associated macrophages. In vivo, one of the major cellular 
      sources of MCP-1 are the smooth muscle cells. As MCP-1 gene expression and/or 
      protein production in these cells is not necessarily correlated with the 
      accumulation of inflammatory cells, there might possibly be additional functions 
      of this cytokine. In the present study, we investigated by use of 35S-labeled 
      antisense RNA probes whether the MCP-1 gene is expressed in tissue specimens of 
      benign prostatic hyperplasia (n = 13) and specimens of prostate carcinoma (n = 
      8), both of which are characterized by a prominent fibromuscular stroma and 
      inconspicuous inflammatory infiltrates. MCP-1 transcripts were located in stromal 
      smooth muscle cells and, additionally, in basal cells of benign prostatic glands. 
      In prostate carcinoma, the number of MCP-1 mRNA-expressing cells was 
      significantly less than in benign prostatic hyperplasia. MCP-1 transcripts were 
      located in preserved fibromuscular stroma and in basal cells of entrapped 
      non-neoplastic glands but not in carcinomatous cells. Immunohistochemical 
      staining with polyclonal antibodies raised against MCP-1 revealed strong 
      reactivity in the fibromuscular stroma surrounding both benign and malignant 
      glands. MCP-1 gene expression or immunoreactivity for anti-MCP-1 antibodies was 
      not related to the rare, lymphocytic interstitial infiltrates. The results show 
      that 1) in the absence of significant leukocyte accumulation, it is unlikely that 
      MCP-1 exerts chemotactic functions in the prostate and 2) that MCP-1, in contrast 
      to previous findings in a wide variety of other human neoplasms, is not expressed 
      in carcinomatous cells of the prostate.
FAU - Mazzucchelli, L
AU  - Mazzucchelli L
AD  - Institute of Pathology, University of Bern, Switzerland.
FAU - Loetscher, P
AU  - Loetscher P
FAU - Kappeler, A
AU  - Kappeler A
FAU - Uguccioni, M
AU  - Uguccioni M
FAU - Baggiolini, M
AU  - Baggiolini M
FAU - Laissue, J A
AU  - Laissue JA
FAU - Mueller, C
AU  - Mueller C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Adult
MH  - Aged
MH  - Cell Count
MH  - Chemokine CCL2/biosynthesis/*genetics
MH  - Gene Expression
MH  - Humans
MH  - Hyperplasia/genetics/pathology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Prostatic Hyperplasia/*genetics/pathology
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - RNA Probes
MH  - RNA, Messenger/*analysis
PMC - PMC1865321
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
PMCR- 1997/02/01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
PHST- 1997/02/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1996 Aug;149(2):501-9.