PMID- 8702980
OWN - NLM
STAT- MEDLINE
DCOM- 19961010
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 271
IP  - 36
DP  - 1996 Sep 6
TI  - Differential regulation of G-protein-mediated signaling by chemokine receptors.
PG  - 21814-9
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a member of a family of chemotactic 
      cytokines that induce directed migration of leukocytes via activation of 
      seven-transmembrane domain receptors. To identify G-proteins that couple to the 
      two forms of the MCP-1 receptor, as well as to related chemokine receptors, we 
      have performed cotransfection experiments in mammalian cells. In COS-7 cells, the 
      type A and type B MCP-1 receptors coupled to Galphai, Galphaq, and Galpha16, 
      whereas the macrophage inflammatory protein-1alpha/RANTES (regulated on 
      activation, normal T cell-expressed and secreted) receptor (C-CR1) coupled to 
      Galphai and Galphaq but failed to couple to Galpha16. In HEK-293 cells, however, 
      the MCP-1 receptors and C-CR1 coupled to Galphaq but failed to couple to 
      Galpha16. In contrast, the interleukin-8 and C5a receptors did not couple to 
      Galphaq in either COS-7 or HEK-293 cells but did couple to Galpha16. Exchange of 
      intracellular loops between the MCP-1 and interleukin-8 receptors to create 
      chimeric receptors revealed that the third loop of the MCP-1 receptor accounted 
      for virtually all of the coupling to Galphaq. We conclude that the MCP-1 and 
      related chemokine receptors couple to multiple G-proteins, that coupling is cell 
      type-specific, and that the third intracellular loop of the C-C type receptors 
      mediates Galphaq coupling.
FAU - Arai, H
AU  - Arai H
AD  - Gladstone Institute of Cardiovascular Disease, University of California, San 
      Francisco, California 94141-9100, USA.
FAU - Charo, I F
AU  - Charo IF
LA  - eng
GR  - HL52773/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Inositol Phosphates)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Receptor, Anaphylatoxin C5a)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Complement)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-8A)
RN  - 0 (Virulence Factors, Bordetella)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.15 (beta-Adrenergic Receptor Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, CD/metabolism
MH  - Cell Line
MH  - Chemokine CCL2/*metabolism
MH  - Chemokine CCL5/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Humans
MH  - Hydrolysis
MH  - Inositol Phosphates/metabolism
MH  - Molecular Sequence Data
MH  - Phosphatidylinositols/metabolism
MH  - Protein Structure, Secondary
MH  - Receptor, Anaphylatoxin C5a
MH  - Receptors, CCR2
MH  - *Receptors, Chemokine
MH  - Receptors, Complement/metabolism
MH  - Receptors, Cytokine/*metabolism
MH  - Receptors, Interleukin/metabolism
MH  - Receptors, Interleukin-8A
MH  - *Signal Transduction
MH  - Structure-Activity Relationship
MH  - Substrate Specificity
MH  - Virulence Factors, Bordetella/pharmacology
MH  - beta-Adrenergic Receptor Kinases
EDAT- 1996/09/06 00:00
MHDA- 1996/09/06 00:01
CRDT- 1996/09/06 00:00
PHST- 1996/09/06 00:00 [pubmed]
PHST- 1996/09/06 00:01 [medline]
PHST- 1996/09/06 00:00 [entrez]
AID - S0021-9258(19)61864-8 [pii]
AID - 10.1074/jbc.271.36.21814 [doi]
PST - ppublish
SO  - J Biol Chem. 1996 Sep 6;271(36):21814-9. doi: 10.1074/jbc.271.36.21814.