PMID- 8704231
OWN - NLM
STAT- MEDLINE
DCOM- 19960912
LR  - 20231213
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 3
DP  - 1996 Aug 1
TI  - TEL-AML1 translocations with TEL and CDKN2 inactivation in acute lymphoblastic 
      leukemia cell lines.
PG  - 785-94
AB  - The t(12;21) (p 13; q22) results in the fusion of the TEL gene located on 
      chromosome 12 with the AML1 gene located on the derivative chromosome 21. Because 
      this translocation is difficult to detect using standard cytogenetic techniques, 
      27 previously karyotyped B-lineage acute lymphoblastic leukemia (ALL) cell lines 
      were evaluated for the presence of the TEL-AML1 fusion using the reverse 
      transcriptase-polymerase chain reaction (RT-PCR), fluorescence in situ 
      hybridization (FISH), and cDNA sequencing. Six cell lines expressed the TEL-AML1 
      chimeric transcript by RT-PCR and the t(12;21) was confirmed by FISH analysis 
      with probes for TEL, AML1, and chromosome 12. While only one of the 6 cell lines 
      with the t(12;21) lost the der(12)t(12;21)-encoded AML1-TEL fusion transcript, 4 
      cell lines lacked expression of the nontranslocated allele of TEL and 5 cell 
      lines lacked expression of CDKN2. Moreover, in 2 patients (1 with the TEL-AML1 
      transcript and 1 without), TEL expression was lost with disease progression; le, 
      TEL was expressed in the initial cell lines (established at diagnosis or first 
      relapse) whereas TEL was not expressed in the cell lines established from these 
      patients in late-stage disease. These data show the coexistence of multiple 
      genetic defects in childhood B-lineage ALL Cell lines with t(12;21) will 
      facilitate the study of TEL-AML1 and AML1-TEL fusion proteins as well as TEL and 
      CDKN2 gene inactivation in leukemia transformation and progression.
FAU - Kim, D H
AU  - Kim DH
AD  - Department of Pediatrics, University of Chicago IL, USA.
FAU - Moldwin, R L
AU  - Moldwin RL
FAU - Vignon, C
AU  - Vignon C
FAU - Bohlander, S K
AU  - Bohlander SK
FAU - Suto, Y
AU  - Suto Y
FAU - Giordano, L
AU  - Giordano L
FAU - Gupta, R
AU  - Gupta R
FAU - Fears, S
AU  - Fears S
FAU - Nucifora, G
AU  - Nucifora G
FAU - Rowley, J D
AU  - Rowley JD
LA  - eng
GR  - CA40046/CA/NCI NIH HHS/United States
GR  - CA42557/CA/NCI NIH HHS/United States
GR  - CA67189/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Carrier Proteins)
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (RUNX1 protein, human)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Base Sequence
MH  - Burkitt Lymphoma/*genetics/pathology
MH  - Carrier Proteins/biosynthesis/*genetics
MH  - Child
MH  - Chromosomes, Human, Pair 12/*genetics/ultrastructure
MH  - Chromosomes, Human, Pair 21/*genetics/ultrastructure
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Cyclin-Dependent Kinase Inhibitor p16
MH  - DNA, Neoplasm/genetics
MH  - DNA-Binding Proteins/biosynthesis/genetics/*metabolism
MH  - Disease Progression
MH  - *Gene Expression Regulation, Leukemic
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/pathology
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-ets
MH  - *Repressor Proteins
MH  - Transcription Factors/biosynthesis/*genetics/*metabolism
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
MH  - ETS Translocation Variant 6 Protein
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - S0006-4971(20)62499-5 [pii]
PST - ppublish
SO  - Blood. 1996 Aug 1;88(3):785-94.