PMID- 8704242
OWN - NLM
STAT- MEDLINE
DCOM- 19960912
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 88
IP  - 3
DP  - 1996 Aug 1
TI  - Direct synergistic effects of leukemia inhibitory factor on hematopoietic 
      progenitor cell growth: comparison with other hematopoietins that use the gp130 
      receptor subunit.
PG  - 863-9
AB  - Because leukemia inhibitory factor (LIF) has little or no effect on murine 
      hematopoietic progenitor cell growth yet enhances hematopoiesis in vivo, we 
      sought to determine whether the effects of LIF were directly or indirectly 
      mediated, or a combination of both. Although LIF alone or in combination with 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3) 
      has no effect on colony formation of unfractionated bone marrow cells (BMCs), it 
      enhances M-CSF-induced colony formation. In comparison, LIF synergizes with IL-3, 
      GM-CSF, M-CSF, and Steel Factor (SLF) to promote the colony formation of 
      partially purified lineage-negative (Lin-) BM progenitors without altering their 
      differentiation. These effects were directly mediated since identical results 
      were observed in single-cell assays. Comparing the effect of LIF with other 
      members of this subclass of hematopoietins (IL-6, oncostatin M [OSM], and ciliary 
      neurotrophic factor [CNTF]), we found that while LIF and IL-6 equally synergize 
      with M-CSF and SLF to promote the colony formation of Lin- BMCs, OSM, and CNTF 
      have no effect. In agreement with OSMs ability to directly bind gp130, 
      preincubation of BMCs with OSM inhibits progenitor cell growth stimulated by the 
      combination of LIF or IL-6 plus SLF. LIF can also directly enhance the growth of 
      further purified more primitive Lin- c-kit+ progenitor cells in the presence of 
      IL-3, GM-CSF, or SLF. Thus, LIF can directly synergize with growth factors to 
      promote the proliferation of purified hematopoietic progenitors, suggesting that 
      the direct effects of LIF on hematopoietic cell growth can, in part, explain the 
      observed hematopoietic effects in vivo. This is a US government work. There are 
      no restrictions on its use.
FAU - Keller, J R
AU  - Keller JR
AD  - Biological Carcinogenesis and Development Program, Science Applications 
      International Corporation Frederick, MD, USA.
FAU - Gooya, J M
AU  - Gooya JM
FAU - Ruscetti, F W
AU  - Ruscetti FW
LA  - eng
GR  - N01-CO-56000/CO/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Growth Inhibitors)
RN  - 0 (Hematopoietic Cell Growth Factors)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Il6st protein, mouse)
RN  - 0 (Interleukin-3)
RN  - 0 (Interleukin-6)
RN  - 0 (LIF protein, human)
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Lif protein, mouse)
RN  - 0 (Lymphokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (OSM protein, human)
RN  - 0 (Osm protein, mouse)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 106956-32-5 (Oncostatin M)
RN  - 133483-10-0 (Cytokine Receptor gp130)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*drug effects/physiology
MH  - Bone Marrow Cells
MH  - Cell Division/drug effects
MH  - Ciliary Neurotrophic Factor
MH  - Colony-Forming Units Assay
MH  - Cytokine Receptor gp130
MH  - Drug Synergism
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Growth Inhibitors/*pharmacology
MH  - Hematopoietic Cell Growth Factors/*pharmacology
MH  - Hematopoietic Stem Cells/*drug effects
MH  - Humans
MH  - Interleukin-3/pharmacology
MH  - *Interleukin-6
MH  - Leukemia Inhibitory Factor
MH  - Lymphokines/*pharmacology
MH  - Macrophage Colony-Stimulating Factor/pharmacology
MH  - Membrane Glycoproteins/*drug effects/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nerve Tissue Proteins/pharmacology
MH  - Oncostatin M
MH  - Peptides/pharmacology
MH  - Proto-Oncogene Proteins c-kit/analysis
MH  - Recombinant Proteins/pharmacology
MH  - Stem Cell Factor/pharmacology
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - S0006-4971(20)62509-5 [pii]
PST - ppublish
SO  - Blood. 1996 Aug 1;88(3):863-9.