PMID- 8704970
OWN - NLM
STAT- MEDLINE
DCOM- 19960911
LR  - 20210316
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 142 ( Pt 5)
DP  - 1996 May
TI  - Molecular characterization of adiY, a regulatory gene which affects expression of 
      the biodegradative acid-induced arginine decarboxylase gene (adiA) of Escherichia 
      coli.
PG  - 1311-1320
LID - 10.1099/13500872-142-5-1311 [doi]
AB  - A transcriptional regulator gene, designated adiY, was found downstream of the 
      biodegradative arginine decarboxylase (adiA) gene (previously known as adi) of 
      Escherichia coli. The arginine decarboxylase system is maximally induced under 
      conditions of acidic pH, anaerobiosis and rich medium, and AdiY was found to 
      increase the expression of adiA. The DNA sequence of adiY encodes a protein of 
      253 amino acids. Primer extension analysis defined the promoter. The amino acid 
      sequence of AdiY showed homology to the XylS/AraC family of transcriptional 
      regulators, which includes EnvY and AppY. Studies suggested that sequences 
      required for acid induction were also necessary to observe the stimulation by 
      AdiY. An examination of the substitution of AdiY, AppY and EnvY showed that these 
      three proteins can, to some extent, stimulate the other systems.
FAU - Stim-Herndon, Kathleen P
AU  - Stim-Herndon KP
AD  - Department of Biochemistry and Cell Biology-MS 140, Rice University, 6100 Main 
      Street, Houston, TX 77005-1892, USA.
FAU - Flores, Theresa M
AU  - Flores TM
AD  - Department of Biochemistry and Cell Biology-MS 140, Rice University, 6100 Main 
      Street, Houston, TX 77005-1892, USA.
FAU - Bennett, George N
AU  - Bennett GN
AD  - Department of Biochemistry and Cell Biology-MS 140, Rice University, 6100 Main 
      Street, Houston, TX 77005-1892, USA.
LA  - eng
SI  - GENBANK/M24530
SI  - GENBANK/U02384
SI  - GENBANK/U02385
SI  - GENBANK/U14003
SI  - GENBANK/X13548
SI  - GENBANK/X58464
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (AdiY protein, E coli)
RN  - 0 (AppY protein, E coli)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EnvY protein, E coli)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 4.1.1.- (Carboxy-Lyases)
RN  - EC 4.1.1.19 (arginine decarboxylase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anaerobiosis
MH  - Bacterial Proteins/biosynthesis/*genetics
MH  - Base Sequence
MH  - Carboxy-Lyases/biosynthesis/*genetics
MH  - Culture Media/pharmacology
MH  - DNA, Bacterial/genetics
MH  - *DNA-Binding Proteins
MH  - Enzyme Induction
MH  - *Escherichia coli Proteins
MH  - *Gene Expression Regulation, Bacterial
MH  - *Genes, Bacterial
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Open Reading Frames
MH  - Promoter Regions, Genetic
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Trans-Activators/genetics/metabolism
MH  - Transcription Factors/classification/*genetics/metabolism
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1099/13500872-142-5-1311 [doi]
PST - ppublish
SO  - Microbiology (Reading). 1996 May;142 ( Pt 5):1311-1320. doi: 
      10.1099/13500872-142-5-1311.