PMID- 8706718
OWN - NLM
STAT- MEDLINE
DCOM- 19960910
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 239
IP  - 1
DP  - 1996 Jul 1
TI  - Follitropin action on the transferrin gene in Sertoli cells is mediated by 
      cAMP-responsive-element-binding-protein and antagonized by chicken 
      ovalbumin-upstream-promoter-transcription factor.
PG  - 52-60
AB  - The transcription of the transferrin (Tf) gene is induced by follitropin via cAMP 
      in rat Sertoli cells. We previously demonstrated that the 
      cAMP-responsive-element-binding protein (CREB) interacts on the proximal region 
      II (PRII) of the human Tf promoter (Suire et al., 1995). The PRII region is 
      identified as essential for cAMP inducibility of the Tf promoter and contains a 
      CCAAT box. This unexpected result led us to study the relation that exists 
      between CREB and the PRII site. In the liver, CCAAT/enhancer-binding (C/EBP) 
      proteins act at the PRII site. Although these factors are absent in Sertoli 
      cells, their overexpression in Sertoli cells disturbs basal and induced 
      transcription. C/EBP alpha and delta were able to stimulate the basal 
      transcription driven by the -100 to +39 region, placed upstream of the 
      chloramphenicol acetyltransferase (CAT) gene. However, only C/EBP alpha allowed 
      the cAMP-inducible expression. The Ka of CREB bZIP (254-327), a deleted form of 
      CREB, for the CRE site (3.92 x 10(8)M-1) and for the PRII site (1.38 x 10(8)M-1) 
      were determined using the surface plasmon resonance (SPR) method. The Ka values 
      were similar, although the derived kinetics were different: higher ka and kd of 
      CREB for the PRII site were found compared with the CRE site. Since we observed 
      important dissociation kinetics, we hypothesized that the binding of CREB to the 
      PRII site is stabilized by CREB-binding protein (CBP) or by 
      chicken-ovalbumin-upstream-promoter transcription factor (COUP-TF) binding to PRI 
      site near to PRII. However, we observed that the overexpression of CBP in Sertoli 
      cells did not potentiate the basal and cAMP-stimulated activity of CREB of the 
      -100 to +39Tf-CAT construct. In basal and cAMP-stimulated conditions, COUP-TF 
      appeared to repress the transcription driven by the -100 to +39 region in a 
      specific manner. These results demonstrate a direct action of CREB on hTf 
      promoter, which is antagonized by COUP-TF and may explain the transcriptional 
      regulation of Tf by follitropin, via cAMP.
FAU - Suire, S
AU  - Suire S
AD  - Institut National de la Recherche Agronomique Station de Physiologie de la 
      Reproduction des Mammiferes Domestiques, INRA/CNRS URA 1291, Nouzilly, France.
FAU - Maurel, M C
AU  - Maurel MC
FAU - Guillou, F
AU  - Guillou F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (COUP Transcription Factor I)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NR2F1 protein, human)
RN  - 0 (Nr2f1 protein, rat)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Transcription Factors)
RN  - 0 (Transferrin)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Bucladesine/antagonists & inhibitors
MH  - COUP Transcription Factor I
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Follicle Stimulating Hormone/antagonists & inhibitors/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Kinetics
MH  - Male
MH  - Oligodeoxyribonucleotides
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Rats
MH  - Rats, Wistar
MH  - Sertoli Cells/*drug effects/metabolism
MH  - Spectrum Analysis
MH  - Transcription Factors/*metabolism
MH  - Transferrin/*genetics
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1111/j.1432-1033.1996.0052u.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1996 Jul 1;239(1):52-60. doi: 10.1111/j.1432-1033.1996.0052u.x.