PMID- 8707716
OWN - NLM
STAT- MEDLINE
DCOM- 19960912
LR  - 20190512
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 74
IP  - 3
DP  - 1996 Mar
TI  - N-methyl-D,L-aspartate-induced growth hormone secretion in barrows: possible 
      mechanisms of action.
PG  - 597-602
AB  - Four experiments were conducted to determine mechanisms by which 
      n-methyl-d,l-aspartate (NMA) increases serum concentrations of growth hormone 
      (GH). Blood samples were collected from barrows every 15 min for 2 h (Exp. 1, 2, 
      and 3) or 3 h (Exp. 4) immediately before and immediately after i.v. treatments. 
      In Exp. 1, barrows (n = 4/treatment) received either .9% saline or 1.25, 2.5, or 
      5 mg of NMA/kg of BW. The change in circulating GH concentrations was greater (P 
      < .05) for barrows receiving 2.5 mg (by 883%) or 5.0 mg of NMA/kg of BW (by 
      1,095%) than for those injected with saline. In Exp. 2, barrows (n = 4/treatment) 
      received NMA (2.5 mg/kg of BW) or injections of 1.25 mg of the pure d or pure 1 
      isomers of NMA/kg of BW. Growth hormone concentrations increased by 177% (P < 
      .025) after NMA treatment and by 245% (P < .01) after injection of the pure d 
      isomer of NMA. The pure 1 isomer of NMA had no effect (P > .1) on GH 
      concentrations. In Exp. 3, barrows received NMA (2.5 mg/kg of BW) 10 min after 
      i.m. injection of saline (n = 7) or ketamine hydrochloride ( n = 8; 19.9 mg/kg of 
      BW), an n-methyl-d-aspartate (NMDA) receptor antagonist. The NMA increased (P < 
      .01) GH concentrations by 289% in saline-pretreated barrows but had no effect (P 
      > .1) on barrows pretreated with ketamine hydrochloride. In Exp. 4, barrows (n = 
      4/treatment) received NMA 3 h after i.v. pretreatment with antisera to 
      GH-releasing factor (GRF; 154 mL) or no pretreatment. Serum GH concentrations 
      increased by 166% (P < .05) after injection of NMA in barrows receiving no 
      pretreatment. The NMA had no effect (P > .1) on GH concentrations in barrows 
      receiving antisera to GRF. Our results support the concept that NMDA stimulates 
      GRF, and hence GH secretion, by activating an NMDA receptor.
FAU - Estienne, M J
AU  - Estienne MJ
AD  - Department of Agriculture, University of Maryland Eastern Shore, Princess Anne 
      21853, USA.
FAU - Harter-Dennis, J M
AU  - Harter-Dennis JM
FAU - Barb, C R
AU  - Barb CR
FAU - Hartsock, T G
AU  - Hartsock TG
FAU - Campbell, R M
AU  - Campbell RM
FAU - Armstrong, J D
AU  - Armstrong JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Immune Sera)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 690G0D6V8H (Ketamine)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Excitatory Amino Acid Agonists/administration & dosage/chemistry/*pharmacology
MH  - Growth Hormone/blood/*metabolism
MH  - Growth Hormone-Releasing Hormone/immunology/physiology
MH  - Immune Sera/immunology/pharmacology
MH  - Injections, Intravenous/veterinary
MH  - Isomerism
MH  - Ketamine/pharmacology
MH  - Male
MH  - N-Methylaspartate/administration & dosage/chemistry/*pharmacology
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/physiology
MH  - Swine/*blood/physiology
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.2527/1996.743597x [doi]
PST - ppublish
SO  - J Anim Sci. 1996 Mar;74(3):597-602. doi: 10.2527/1996.743597x.