PMID- 8708409
OWN - NLM
STAT- MEDLINE
DCOM- 19960909
LR  - 20190821
IS  - 0387-5911 (Print)
IS  - 0387-5911 (Linking)
VI  - 69
IP  - 11
DP  - 1995 Nov
TI  - Predominance of codon 215 mutation in reverse transcriptase-coding region of 
      3'-azido-3'-deoxythymidine (AZT)-resistant HIV-1 isolates after long-term AZT 
      therapy.
PG  - 1278-85
AB  - Among human immunodeficiency type 1 viruses (HIV-1) isolated during long-term 
      3'-azido-3'-deoxythymidine (AZT) therapy, 4 condoms (Asp67, Lys70, Thr215, and 
      Lys219) in the HIV-1-reverse transcriptase (RT)-coding region have been 
      considered to be related to the AZT resistance of HIV-1. Therefore we determined 
      these mutation patterns in HIV-1 isolates from patients undergoing long-term AZT 
      treatment. In 41 clones of HIV-1 from 7 patients, the Thr215 mutation was the 
      most predominant (97.6%), and more frequent than Asp67 (48.8%), Lys70 (31.7%) and 
      Lys219 (9.8%) mutations. All 22 clones from isolates cultured in the presence of 
      1 microM AZT Thr215 mutation; such a high frequency was not found for the other 3 
      codon mutations. In a clinical follow-up study, Thr215 mutation appeared in the 
      late stage of AZT treatment parallel with the emergence of AZT insusceptibility. 
      It is worth noting that this Thr215 mutation to Tyr or Phe is a 2-nucleotide 
      mutation in contrast to the 1-nucleotide mutations seen in the other 3 codons. 
      Isolates with the single amino acid change of Thr215 of the RT-coding region 
      obtained after long-term AZT treatment grew in the presence of 1 microM AZT. This 
      single amino acid mutation in the Thr215 codon is the most important factor in 
      AZT resistance.
FAU - Kondo, M
AU  - Kondo M
AD  - Department of Virology, Kanagawa Prefectural Public Health Laboratory, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Kansenshogaku Zasshi
JT  - Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious 
      Diseases
JID - 0236671
RN  - 0 (Amino Acids)
RN  - 0 (Antiviral Agents)
RN  - 0 (Codon)
RN  - 4B9XT59T7S (Zidovudine)
RN  - EC 2.7.7.49 (HIV Reverse Transcriptase)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*drug therapy/*virology
MH  - Amino Acids/genetics
MH  - Antiviral Agents/*administration & dosage
MH  - Codon/*genetics
MH  - Drug Resistance/genetics
MH  - HIV Reverse Transcriptase
MH  - HIV-1/*genetics
MH  - Humans
MH  - Mutation
MH  - RNA-Directed DNA Polymerase/*genetics
MH  - Zidovudine/*administration & dosage
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.11150/kansenshogakuzasshi1970.69.1278 [doi]
PST - ppublish
SO  - Kansenshogaku Zasshi. 1995 Nov;69(11):1278-85. doi: 
      10.11150/kansenshogakuzasshi1970.69.1278.