PMID- 8712509
OWN - NLM
STAT- MEDLINE
DCOM- 19960909
LR  - 20191210
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 57
IP  - 4
DP  - 1996 Apr
TI  - Cytokine release by porcine livers perfused with lipopolysaccharide or live 
      Salmonella choleraesuis.
PG  - 472-6
AB  - OBJECTIVE: To develop a model to study the kinetics and relative amounts of 
      cytokines produced by liver cells during enteric infection. DESIGN: Salmonella 
      enteriditis lipopolysaccharide (LPS)- or live S choleraesuis-stimulated isolated 
      livers from clinically normal pigs and pigs with active acute phase response. 
      ANIMALS: 7- to 14-day-old salmonellosis-free pigs, 4 to 12/group. PROCEDURE: 
      Livers were removed and perfused with oxygenated Krebs-Henseleit solution for 30 
      minutes and with S choleraesuis or LPS added for 7 minutes. Livers were then 
      perfused with 500 ml of fresh solution in a closed loop procedure for 180 
      minutes. Perfusate samples were collected for tumor necrosis factor-alpha (TNF 
      alpha) and interleukin 6 (IL-6) bioassays. RESULTS: Tumor necrosis factor-alpha 
      values remained constant during perfusion of normal livers and increased in those 
      exposed to LPS. Interleukin 6 values increased in perfusate from normal livers 
      from 30 to 150 minutes, then decreased. In livers from pigs with an active acute 
      phase response, TNF alpha values were reduced; IL-6 appeared by 2 minutes and 
      decreased after 25 minutes. CONCLUSIONS: Isolated livers could be kept viable for 
      3 hours, and IL-6 and TNF alpha could be measured by the bioassays used. CLINICAL 
      RELEVANCE: Model can be used for studying and modifying the response of liver 
      cells to infective agents.
FAU - Frank, D E
AU  - Frank DE
AD  - Department of Microbiology, Immunology, and Preventive Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames 50011, USA.
FAU - Ahrens, F A
AU  - Ahrens FA
FAU - Kramer, T T
AU  - Kramer TT
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Biological Assay
MH  - Cell Survival/drug effects
MH  - Glucose/analysis
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-6/analysis/*metabolism/pharmacology
MH  - Kinetics
MH  - L Cells
MH  - L-Lactate Dehydrogenase
MH  - Lipopolysaccharides/*pharmacology
MH  - Liver/cytology/drug effects/*immunology
MH  - Mice
MH  - Perfusion
MH  - Potassium/analysis
MH  - Salmonella/*immunology
MH  - Swine
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/analysis/*metabolism/pharmacology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
PST - ppublish
SO  - Am J Vet Res. 1996 Apr;57(4):472-6.