PMID- 8717152
OWN - NLM
STAT- MEDLINE
DCOM- 19961022
LR  - 20190726
IS  - 0028-1298 (Print)
IS  - 0028-1298 (Linking)
VI  - 353
IP  - 2
DP  - 1996 Jan
TI  - The effect of endothelin-2 (ET-2) on migration and changes in cytosolic free 
      calcium of neutrophils.
PG  - 130-5
AB  - The effect of endothelin-2 (ET-2) on neutrophil migration and intracellular 
      calcium was studied. Depending on the concentration, ET-2 enhanced or inhibited 
      neutrophil migration. At low concentrations ET-2 caused a chemotactic stimulation 
      of migration, in contrast with endothelin-1 (ET-1) which caused a chemokinetic 
      stimulation of migration. At higher concentrations ET-2 inhibited 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-activated migration. Both activation 
      and inhibition by ET-2 were completely dependent on extracellular Ca2+. Unlike 
      ET-1 which caused an increase in cytosolic free Ca2+ at a concentration which 
      stimulated migration, ET-2 caused a measurable increase of cytosolic free Ca2+ at 
      a concentration which did not stimulate migration. This strongly suggests that 
      there is no correlation between maximal stimulation of cytoplasmic free calcium, 
      and maximal stimulation of migration. Influx of extracellular Ca2+ was required 
      for both activation of migration and change in cytosolic free Ca2+, because no 
      effect was observed in the absence of extracellular Ca2+, and because blockers of 
      Ca(2+)-influx inhibited ET-2-activated migration. The ETA-receptor antagonist 
      cyclo-(-D-Trp-D-Asp-Pro-D-Val-Leu) (BQ123), and the ETB-receptor antagonist 
      [Cys11-Cys15]-endothelin-1(11-21) (IRL1038) antagonized the stimulatory effect of 
      ET-2 on migration, and the inhibitory effect of high concentrations of ET-2 on 
      fMLP-activated chemotaxis. This suggests that both the ETA-receptor and the 
      ETB-receptor are involved in the stimulatory effect of low concentrations of 
      ET-2, and in the inhibitory effect of high concentrations of ET-2.
FAU - Elferink, J G
AU  - Elferink JG
AD  - Department of Medical Biochemistry, University of Leiden, Netherlands.
FAU - de Koster, B M
AU  - de Koster BM
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Naunyn Schmiedebergs Arch Pharmacol
JT  - Naunyn-Schmiedeberg's archives of pharmacology
JID - 0326264
RN  - 0 (Endothelins)
RN  - CJ0O37KU29 (Verapamil)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Chemotaxis/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endothelins/*pharmacology
MH  - Neutrophils/*drug effects
MH  - Rabbits
MH  - Verapamil/pharmacology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF00168749 [doi]
PST - ppublish
SO  - Naunyn Schmiedebergs Arch Pharmacol. 1996 Jan;353(2):130-5. doi: 
      10.1007/BF00168749.