PMID- 8719877
OWN - NLM
STAT- MEDLINE
DCOM- 19970306
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 108 ( Pt 12)
DP  - 1995 Dec
TI  - The hepatocyte growth factor/scatter factor (HGF/SF) receptor, met, transduces a 
      morphogenetic signal in renal glomerular fibromuscular mesangial cells.
PG  - 3703-14
AB  - Previous studies have demonstrated that hepatocyte growth factor/scatter factor 
      (HGF/SF) is secreted by mesenchymal cells and that it elicits motility, 
      morphogenesis and proliferation of epithelia expressing the met receptor. We now 
      report that HGF/SF may act as an autocrine factor in fibromuscular renal 
      mesangial cells. These cells mechanically support glomerular endothelia, control 
      the rate of plasma ultrafiltration and are implicated in the pathogenesis of a 
      variety of chronic renal diseases. We detected met protein in the vascular stalk 
      of metanephric glomeruli and in the mature mesangium. Mesangial lines from a 
      mouse transgenic for a temperature-sensitive simian virus 40 T antigen expressed 
      met mRNA and protein, and recombinant HGF/SF phosphorylated the met receptor 
      tyrosine kinase. Cells were immortal in the permissive condition and HGF/SF 
      enhanced proliferation in a defined medium. In the absence of the immortalising 
      protein, division ceased and recombinant HGF/SF caused multipolar cells to become 
      bipolar. The factor diminished stress fibres, their focal contacts and 
      immunostaining for extracellular fibronectin, hence suggesting reduced substratum 
      adhesion and enhanced motility. Mesangial lines also expressed HGF/SF mRNA and 
      secreted bioactive factor; immunocytochemistry showed both ligand and receptor in 
      individual cells. HGF/SF blocking antibody aggregated the cells, suggesting that 
      mesangial-derived factor affects basal cell conformation in an autocrine manner. 
      We conclude that mesangial cells express both HGF/SF and met, and the factor 
      induces morphogenesis of cultured mesangial cells. Therefore HGF/SF may have an 
      autocrine role in mesangial biology but further studies are now required to 
      investigate the potential importance of the factor in vivo.
FAU - Kolatsi-Joannou, M
AU  - Kolatsi-Joannou M
AD  - Developmental Biology Unit, Institute of Child Health, London, UK.
FAU - Woolf, A S
AU  - Woolf AS
FAU - Hardman, P
AU  - Hardman P
FAU - White, S J
AU  - White SJ
FAU - Gordge, M
AU  - Gordge M
FAU - Henderson, R M
AU  - Henderson RM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Glomerular Mesangium/chemistry/cytology/*growth & development
MH  - *Hepatocyte Growth Factor
MH  - Immunohistochemistry
MH  - Mice
MH  - Morphogenesis
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/analysis/*physiology
MH  - Signal Transduction/*physiology
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1242/jcs.108.12.3703 [doi]
PST - ppublish
SO  - J Cell Sci. 1995 Dec;108 ( Pt 12):3703-14. doi: 10.1242/jcs.108.12.3703.