PMID- 8720686
OWN - NLM
STAT- MEDLINE
DCOM- 19961008
LR  - 20191101
IS  - 1016-0922 (Print)
IS  - 1016-0922 (Linking)
VI  - 4
IP  - 4
DP  - 1995 Jul-Aug
TI  - Properties of intracellular calcium stores and their role in receptor-mediated 
      catecholamine secretion in rat adrenal chromaffin cells.
PG  - 195-205
AB  - Simulation of rat adrenal chromaffin cells either with receptor agonists such as 
      muscarine, bradykinin and histamine or with caffeine in Ca(2+)-free Krebs 
      solution induced a brief increase in the intracellular free Ca2+ concentration, 
      [Ca2+]i, which terminated within 90 s. Both the agonist- and caffeine-induced 
      [Ca2+]i responses were abolished in cells which had been treated with either 500 
      nM thapsigargin (TG) or 20 microM ryanodine, suggesting that inositol 
      trisphosphate and caffeine cause Ca2+ release either from the same Ca2+ store or 
      from distinct stores which exchange Ca2+ rapidly. In normal Krebs solution, these 
      agonists evoked catecholamine (CA) secretion which showed an initial transient 
      followed by a sustained component. Neither component of the secretion was 
      significantly affected by TG or ryanodine. In the medium containing 16 microM 
      Ca2+ or no Ca2+, CA secretion evoked by 30-second stimulation with 100 microM 
      muscarine was 59 or 7%, respectively, of that evoked in the normal medium 
      containing 2 mM Ca2+. In TG-treated cells, the CA secretion at 16 microM Ca2+ was 
      reduced to 23% and that in the Ca(2+)-free medium was completely abolished. These 
      results suggest that the receptor-mediated Ca2+ entry solely determines the rate 
      of CA secretion in rat chromaffin cells when stimulated by receptor agonists in 
      the normal medium, whereas intracellular Ca2+ release and Ca2+ entry may 
      cooperatively support the secretion when cells are stimulated in media containing 
      low concentrations of Ca2+.
FAU - Warashina, A
AU  - Warashina A
AD  - Department of Physiology, Niigata University School of Medicine, Japan.
FAU - Fujiwara, N
AU  - Fujiwara N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Biol Signals
JT  - Biological signals
JID - 9210083
RN  - 0 (Catecholamines)
RN  - 0 (Receptors, Cell Surface)
RN  - 15662-33-6 (Ryanodine)
RN  - 3G6A5W338E (Caffeine)
RN  - 7T101UWZ5W (Muscarine)
RN  - 820484N8I3 (Histamine)
RN  - S8TIM42R2W (Bradykinin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adrenal Medulla/drug effects/*physiology
MH  - Animals
MH  - Bradykinin/*pharmacology
MH  - Caffeine/pharmacology
MH  - Calcium/*metabolism
MH  - Catecholamines/*metabolism
MH  - Histamine/*pharmacology
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Male
MH  - Muscarine/*pharmacology
MH  - Perfusion
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cell Surface/agonists
MH  - Ryanodine/pharmacology
MH  - Time Factors
EDAT- 1995/07/01 00:00
MHDA- 1995/07/01 00:01
CRDT- 1995/07/01 00:00
PHST- 1995/07/01 00:00 [pubmed]
PHST- 1995/07/01 00:01 [medline]
PHST- 1995/07/01 00:00 [entrez]
AID - 10.1159/000109443 [doi]
PST - ppublish
SO  - Biol Signals. 1995 Jul-Aug;4(4):195-205. doi: 10.1159/000109443.