PMID- 8721421
OWN - NLM
STAT- MEDLINE
DCOM- 19961002
LR  - 20191024
IS  - 0937-9827 (Print)
IS  - 0937-9827 (Linking)
VI  - 108
IP  - 5
DP  - 1996
TI  - The dynamics of inflammatory cytokines in the healing process of mouse skin 
      wound: a preliminary study for possible wound age determination.
PG  - 231-6
AB  - The dynamics of inflammatory cytokines such as interleukin-1 alpha (IL-1 alpha), 
      interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis 
      factor-alpha (TNF alpha) during the healing process of mouse skin wounds were 
      examined using the enzyme-linked immunosorbent assay and immunostaining. The 
      applicability of this examination for wound age estimation is discussed from the 
      perspective of forensic pathology. After wound induction, mice were sacrificed at 
      intervals ranging from 0 to 240 h. The levels of TNF alpha and IL-1 beta began to 
      elevate rapidly after wounding and reached a peak at 3 h. The IL-1 alpha level 
      reached a peak at 6 h, and IL-6 peaked at 12 h. An infiltration of numerous 
      leukocytes, indicating acute inflammation, was observed at 3 and 6 h, and the 
      main source of the cytokines was immunohistochemically identified as neutrophils. 
      These results indicate that TNF alpha and IL-1 beta play an important role in the 
      commencement of inflammation. "Rebound" of cytokine levels, i.e. a re-increase, 
      was observed at 72 h after wounding. Histological examination of the 72-h-old 
      wound showed migration of fibroblasts and the formation of new granulation 
      tissues, indicating the proliferative stage of the wound healing process. These 
      experimental findings indicate that these cytokines have a close relationship to 
      wound remodeling as well as to inflammation. From the viewpoint of forensic 
      pathology, it is considered that inflammatory cytokines may become one of the 
      markers for wound age estimation, but further studies are needed, especially 
      those involving the investigation using human wound specimens with known time 
      intervals after injury.
FAU - Kondo, T
AU  - Kondo T
AD  - Department of Legal Medicine, Kanazawa University, Faculty of Medicine, Kanazawa, 
      Japan.
FAU - Ohshima, T
AU  - Ohshima T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Int J Legal Med
JT  - International journal of legal medicine
JID - 9101456
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Forensic Medicine
MH  - Immunoenzyme Techniques
MH  - Interleukin-1/*metabolism
MH  - Interleukin-6/*metabolism
MH  - Keratinocytes/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Skin/*injuries/pathology
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Wound Healing/*physiology
MH  - Wounds, Penetrating/*pathology
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF01369816 [doi]
PST - ppublish
SO  - Int J Legal Med. 1996;108(5):231-6. doi: 10.1007/BF01369816.