PMID- 8722634
OWN - NLM
STAT- MEDLINE
DCOM- 19960926
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 54
IP  - 5
DP  - 1996 May
TI  - Prolactin-induced regression of the rat corpus luteum: expression of monocyte 
      chemoattractant protein-1 and invasion of macrophages.
PG  - 1120-7
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a potential mediator of the 
      recruitment of monocytes/macrophages into the regressing corpus luteum (CL). We 
      investigated whether the luteolytic effect of prolactin in the rat is associated 
      with the expression of MCP-1 and an invasion of monocytes/macrophages. Ovulation 
      was induced in immature female rats by injection of eCG (5 IU, s.c.) at 30 days 
      of age. All rats were hypophysectomized 3 days later. Rats received injections of 
      ovine prolactin (250 micrograms, s.c.) at 12-h intervals on Day 9, 10, and 11 
      posthypophysectomy; controls received injection of vehicle. Rats were killed by 
      decapitation 24, 48, or 72 h after the first injection of prolactin or vehicle. 
      In rats treated with prolactin, immunoreactive MCP-1 was detected in the CL at 24 
      h after the first injection, and a consistent level of staining was reached by 72 
      h with immunodetectable MCP-1 diffused throughout individual CL. The number of 
      monocytes/macrophages in the CL (mean +/- SEM) increased significantly after 
      prolactin treatment, from 3.1 +/- 1.8 at 24 h to 49.3 +/- 8.2 at 72 h (p < 0.05), 
      and the number of monocytes/macrophages was different from that in control, 
      vehicle-treated rats at 72 h (10.3 +/- 4.1; p < 24 and 72 h in prolactin-treated 
      rats (p < 0.05). It is concluded that a potentially important component of the 
      luteolytic effect of prolactin in the rat is the expression of MCP-1 and invasion 
      of monocytes/macrophages into the CL.
FAU - Bowen, J M
AU  - Bowen JM
AD  - Department of Physiology, University of Michigan Medical School, Ann Arbor 
      48109-0622, USA.
FAU - Keyes, P L
AU  - Keyes PL
FAU - Warren, J S
AU  - Warren JS
FAU - Townson, D H
AU  - Townson DH
LA  - eng
GR  - HL48287/HL/NHLBI NIH HHS/United States
GR  - T32-HD07048/HD/NICHD NIH HHS/United States
GR  - U54 HD29184/HD/NICHD NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 145-14-2 (20-alpha-Dihydroprogesterone)
RN  - 9002-62-4 (Prolactin)
SB  - IM
EIN - Biol Reprod 1996 Jul;55(1):224
MH  - 20-alpha-Dihydroprogesterone/blood
MH  - Animals
MH  - Chemokine CCL2/analysis/*metabolism
MH  - Corpus Luteum/cytology/*physiology
MH  - Female
MH  - Hypophysectomy
MH  - Immunohistochemistry
MH  - Luteolysis/*drug effects
MH  - Macrophages/*physiology
MH  - Ovulation Induction
MH  - Prolactin/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1095/biolreprod54.5.1120 [doi]
PST - ppublish
SO  - Biol Reprod. 1996 May;54(5):1120-7. doi: 10.1095/biolreprod54.5.1120.