PMID- 8723439
OWN - NLM
STAT- MEDLINE
DCOM- 19961029
LR  - 20220409
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 17
IP  - 2
DP  - 1996 Mar-Apr
TI  - Effect of sperm lipid peroxidation on fertilization.
PG  - 151-7
AB  - This study was aimed at determining, with homologous mouse gametes, whether a 
      level of membrane lipid peroxidation insufficient to affect sperm motility 
      parameters can alter sperm fertilizing potential. The addition of ferrous ions 
      and ascorbic acid (Fe2+/ Asc) to mouse sperm suspensions increases the formation 
      of thiobarbituric acid-reactive substances (TBARS), an indicator of lipid 
      peroxide breakdown products, without significantly affecting the level of 
      sulfhydryl groups. In the presence of Fe2+/Asc concentrations above > 0.4/2.0 mM, 
      spermatozoa become immotile. However, at concentrations < or = 0.4/2.0 mM of 
      Fe2+/Asc, i.e., conditions in which the TBARS formation is increased by < or = 
      4.6-fold over that of controls, motility remains unaffected for up to 3 hours. In 
      the presence of 0.4/ 2.0 mM Fe2+/Asc, treated spermatozoa increase their 
      fertilizing potential by 50%, as measured by the formation of two-cell embryos. 
      This increase is not caused by improvements in sperm motility parameters 
      (percentage, linearity, velocity, hyperactivation) or sperm capacitation. On the 
      other hand, there is a significant increase in the capacity of mouse spermatozoa 
      to bind to homologous zona pellucida. In conclusion, mild peroxidative 
      conditions, that increase lipid peroxide formation 4.6-fold without significantly 
      modifying free sulfhydryl groups and sperm motility parameters, improve the 
      fertilizing potential of spermatozoa by increasing their binding capacity to zona 
      pellucida.
FAU - Kodama, H
AU  - Kodama H
AD  - Urology Research Laboratory, Royal Victoria Hospital, Montreal, Canada.
FAU - Kuribayashi, Y
AU  - Kuribayashi Y
FAU - Gagnon, C
AU  - Gagnon C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Ferrous Compounds)
RN  - 0 (Membrane Lipids)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 39R4TAN1VT (ferrous sulfate)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Ascorbic Acid/pharmacology
MH  - Female
MH  - Ferrous Compounds/pharmacology
MH  - Fertility/drug effects
MH  - Fertilization/*physiology
MH  - Fertilization in Vitro
MH  - *Lipid Peroxidation
MH  - Male
MH  - Membrane Lipids
MH  - Mice
MH  - Sperm Capacitation/drug effects
MH  - Sperm Motility/drug effects
MH  - Sperm-Ovum Interactions/physiology
MH  - Spermatozoa/*metabolism
MH  - Sulfhydryl Compounds/analysis
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Zona Pellucida/drug effects
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PST - ppublish
SO  - J Androl. 1996 Mar-Apr;17(2):151-7.