PMID- 8723904
OWN - NLM
STAT- MEDLINE
DCOM- 19961010
LR  - 20220225
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 24
IP  - 1
DP  - 1996 May 1
TI  - Depletion of intracellular calcium stores facilitates the influx of extracellular 
      calcium in platelet derived growth factor stimulated A172 glioblastoma cells.
PG  - 64-73
AB  - Calcium signaling in non-excitable cells is the consequence of calcium release 
      from intracellular stores, at times followed by entry of extracellular calcium 
      through the plasma membrane. To study whether entry of calcium depends upon the 
      level of saturation of intracellular stores, we measured calcium channel opening 
      in the plasma membrane of single confluent A172 glioblastoma cells stimulated 
      with platelet derived growth factor (PDGF) and/or bradykinin (BK). We monitored 
      the entry of extracellular calcium by measuring manganese quenching of Indo-1 
      fluorescence. PDGF raised intracellular calcium concentration ([Ca2+]i) after a 
      dose-dependent delay (tdel) and then opened calcium channels after a 
      dose-independent delay (tch). At higher doses (> 3 nM), BK increased [Ca2+]i 
      after a tdel approximately 0 s, and tch decreased inversely with both dose and 
      peak [Ca2+]i. Experiments with thapsigargin (TG), BK, and PDGF indicated that BK 
      and PDGF share intracellular Ca2+ pools that are sensitive to TG. When these 
      stores were depleted by treatment with BK and intracellular BAPTA, tdel did not 
      change, but tch fell to almost 0 s in PDGF stimulated cells, indicating that 
      depletion of calcium stores affects calcium channel opening in the plasma 
      membrane. Our data support the capacitative model for calcium channel opening and 
      the steady-state model describing quantal Ca2+ release from intracellular stores.
FAU - Vereb, G Jr
AU  - Vereb G Jr
AD  - Department of Biophysics, University Medical School of Debrecen, Hungary.
FAU - Szollosi, J
AU  - Szollosi J
FAU - Matyus, L
AU  - Matyus L
FAU - Balazs, M
AU  - Balazs M
FAU - Hyun, W C
AU  - Hyun WC
FAU - Feuerstein, B G
AU  - Feuerstein BG
LA  - eng
GR  - CA 13525/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (Terpenes)
RN  - 1B56C968OA (Becaplermin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 6I3K30563S (Lanthanum)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - S8TIM42R2W (Bradykinin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Becaplermin
MH  - Bradykinin/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Dose-Response Relationship, Drug
MH  - Glioblastoma
MH  - Humans
MH  - Lanthanum/pharmacology
MH  - Neuroglia/drug effects/*metabolism
MH  - Platelet-Derived Growth Factor/*pharmacology
MH  - Proto-Oncogene Proteins c-sis
MH  - *Signal Transduction
MH  - Terpenes/pharmacology
MH  - Thapsigargin
MH  - Tumor Cells, Cultured
EDAT- 1996/05/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0320(19960501)24:1<64::AID-CYTO8>3.0.CO;2-I [pii]
AID - 10.1002/(SICI)1097-0320(19960501)24:1<64::AID-CYTO8>3.0.CO;2-I [doi]
PST - ppublish
SO  - Cytometry. 1996 May 1;24(1):64-73. doi: 
      10.1002/(SICI)1097-0320(19960501)24:1<64::AID-CYTO8>3.0.CO;2-I.