PMID- 8724438
OWN - NLM
STAT- MEDLINE
DCOM- 19961204
LR  - 20191101
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 71
IP  - 3
DP  - 1996 May
TI  - Highly selective acridine and ethidium staining of bacterial DNA and RNA.
PG  - 130-6
AB  - The acridine dyes acridine orange (AO) and coriphosphine O (CPO) and ethidium 
      bromide (EtBr) were used to stain bacterial digests after electrophoresis in 
      native and denaturing (SDS) polyacrylamide gels and were shown to stain DNA and 
      RNA preferentially over other subcellular components in the gels. Vegetative cell 
      digests of Bacillus subtilis, Escherichia coli, Micrococcus luteus, and 
      Staphylococcus aureus showed intense staining of DNA with AO and CPO near the top 
      of the gel, but little or no staining of other cellular constituents. EtBr 
      stained both DNA and RNA in the gels. Protein standards and non-nucleic acid 
      cellular constituents stained faintly with high concentrations (> or = 100 
      microM) of AO, lower concentrations (13.9 microM) of CPO, and did not stain with 
      0.5 microgram/ml EtBr in denaturing gels. The complete set of cellular 
      biochemicals was visualized by silver staining, while the protein subset was 
      detected by Coomassie blue staining. The highest concentrations of AO (120 
      microM) and CPO (13.9 microM) were shown to detect purified DNA in gels with a 
      sensitivity in the range of 25-50 ng per band. This work demonstrates the 
      specificity of acridine and ethidium dyes for nucleic acids, while illustrating 
      the level of non-nucleic acid-specific interactions with other cellular 
      components by staining of electrophoretically separated cellular components in a 
      gel matrix.
FAU - Bruno, J G
AU  - Bruno JG
AD  - Armstrong Laboratory, Tyndall Air Force Base, Florida 32403, USA.
FAU - Sincock, S A
AU  - Sincock SA
FAU - Stopa, P J
AU  - Stopa PJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (Aminoacridines)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Bacterial)
RN  - 5153-57-1 (coriphosphine)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Aminoacridines/*chemistry
MH  - Bacillus subtilis/genetics
MH  - DNA, Bacterial/*analysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/genetics
MH  - Ethidium/*chemistry
MH  - Fluorescent Dyes/*chemistry
MH  - Micrococcus luteus/genetics
MH  - Pseudomonas fluorescens/genetics
MH  - RNA, Bacterial/*analysis
MH  - Staining and Labeling
MH  - Staphylococcus aureus/genetics
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.3109/10520299609117149 [doi]
PST - ppublish
SO  - Biotech Histochem. 1996 May;71(3):130-6. doi: 10.3109/10520299609117149.