PMID- 8727882
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 34
IP  - 5
DP  - 1996 May
TI  - Studies of in vivo distribution of bovine herpesvirus type 4 in the natural host.
PG  - 1091-5
AB  - The in vivo distribution of bovine herpesvirus type 4 (BHV-4) was examined by 
      testing nasal and conjunctival exudates, peripheral blood leukocytes, and various 
      organs of experimentally infected calves. For virus detection, a nested PCR 
      assay, virus isolation, and immunohistochemistry were applied. The nervous system 
      and the muscles were free of viral DNA. Liver and intestinal lymph nodes 
      contained low amounts of virus (less than two copies per 1 microgram of cellular 
      DNA). Intestinal, tonsil, thymus, and kidney tissues contained more viral DNA 
      copies (5 to 50 copies per 1 microgram of cellular DNA). The highest amounts of 
      BHV-4 DNA (50 to 500 copies per 1 microgram of cellular DNA) were found in the 
      spleen, lungs, trachea, and nasal epithelium. Amplification of DNA from blood 
      lymphocytes through postinoculation (p.i.) day 48 proved that the virus started 
      to replicate in these cells immediately after inoculation of the calves and that 
      intensive virus growth took place during the 7 to 8 weeks of the infection. The 
      number of virus-infected lymphocytes reached the maximum on p.i. days 22 to 26 
      and slowly declined thereafter. Virus-infected cells were found only in the 
      spleen on p.i. day 48 by immunohistochemistry. Western blotting (immunoblotting) 
      detected signs of an immune response against 9 of the 29 BHV-4 proteins.
FAU - Egyed, L
AU  - Egyed L
AD  - Veterinary Medical Research Institute of the Hungarian Academy of Sciences, 
      Budapest, Hungary.
FAU - Ballagi-Pordany, A
AU  - Ballagi-Pordany A
FAU - Bartha, A
AU  - Bartha A
FAU - Belak, S
AU  - Belak S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Primers)
SB  - IM
CIN - J Clin Microbiol. 1996 Dec;34(12):3253-4. PMID: 8940490
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cattle
MH  - Cattle Diseases/*virology
MH  - DNA Primers/genetics
MH  - Gammaherpesvirinae/genetics/immunology/*isolation & purification
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Polymerase Chain Reaction/methods/statistics & numerical data
MH  - Sensitivity and Specificity
PMC - PMC228961
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
PMCR- 1996/05/01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PHST- 1996/05/01 00:00 [pmc-release]
AID - 10.1128/jcm.34.5.1091-1095.1996 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1996 May;34(5):1091-5. doi: 10.1128/jcm.34.5.1091-1095.1996.