PMID- 8728016
OWN - NLM
STAT- MEDLINE
DCOM- 19970212
LR  - 20190920
IS  - 0360-3997 (Print)
IS  - 0360-3997 (Linking)
VI  - 20
IP  - 2
DP  - 1996 Apr
TI  - Changes of fatty acid composition and oxidative metabolism of human neutrophils 
      migrating into an inflammatory exudate.
PG  - 123-37
AB  - The peripheral blood neutrophils and the neutrophils accumulated into a 
      skin-window experimental exudate were compared for their ability to release 
      superoxide anion (O2-) and for their fatty acid composition, determined by 
      capillary gaschromatography. The basal O2- release and the phorbol myristate 
      acetate (PMA)-induced O2- release were not significantly different in the two 
      neutrophil populations, while in response to 
      formyl-methionyl-leucyl-phenylalanine (fMLP) the exudate cells showed an activity 
      that was two fold higher than that of blood cells. The most significant changes 
      of fatty acid composition of exudate versus blood cells were the following: i) 
      increase of C16:0 (palmitic acid) from 21.3 +/- 1.2% to 23.5 +/- 1.3% (+ 10.2%) 
      of total fatty acids (p < 0.001),ii) increase of C18:2 (linoleic acid) from 9.3 
      +/- 1.7% to 11.0 +/- 2.1% (+ 18.3%) (p = 0.005), iii) decrease of C20:4 
      (arachidonic acid) from 12.8 +/- 1.6% to 10.6 +/- 1.2% (-17.3%) (p < 0.001), 
      whereas C18:1 (oleic acid) did not change. The total content of saturated or 
      unsaturated fatty acids did not change. In exudate cells, a strong negative 
      correlation was found between palmitic acid content and O2- release in response 
      to both fMLP and PMA (r = -0.52, p < 0.02 and r = -0.49, p < 0.05, respectively) 
      whereas arachidonic acid correlated positively, but weakly, with O2- (r = 0.40, p 
      = 0.07 and r = 0.47, p = 0.05, with fMLP and PMA as stimulants respectively). A 
      positive correlation was also found between the arachidonic acid content of blood 
      cells and the number of cells that migrated into the inflammatory exudate. These 
      results indicate that the process of extravasation from blood into the exudate 
      causes specific and consistent modifications of the fatty acid composition of 
      neutrophils and suggest that these modifications have a role in the activation 
      and the regulation of the O2- forming system.
FAU - Carletto, A
AU  - Carletto A
AD  - Institute of Medical Pathology, University of Verona, Italy.
FAU - Bellavite, P
AU  - Bellavite P
FAU - Guarini, P
AU  - Guarini P
FAU - Biasi, D
AU  - Biasi D
FAU - Chirumbolo, S
AU  - Chirumbolo S
FAU - Caramaschi, P
AU  - Caramaschi P
FAU - Bambara, L M
AU  - Bambara LM
FAU - Corrocher, R
AU  - Corrocher R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Fatty Acids)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - *Chemotaxis, Leukocyte/drug effects
MH  - Exudates and Transudates/*cytology
MH  - Fatty Acids/*chemistry/classification
MH  - Humans
MH  - Inflammation
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*metabolism/physiology
MH  - Oxidation-Reduction
MH  - *Respiratory Burst/drug effects
MH  - Skin Window Technique
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1007/BF01487400 [doi]
PST - ppublish
SO  - Inflammation. 1996 Apr;20(2):123-37. doi: 10.1007/BF01487400.