PMID- 8730306
OWN - NLM
STAT- MEDLINE
DCOM- 19961030
LR  - 20190512
IS  - 0035-9203 (Print)
IS  - 0035-9203 (Linking)
VI  - 90
IP  - 1
DP  - 1996 Jan-Feb
TI  - Acridine orange fluorescence techniques as alternatives to traditional Giemsa 
      staining for the diagnosis of malaria in developing countries.
PG  - 34-6
AB  - Traditional Giemsa-stained thick blood films were compared with 2 fluorescence 
      microscopy techniques, acridine orange (AO) staining of thin blood films and the 
      quantitative buffy coat (QBC) method, for the microscopical diagnosis of malaria. 
      Of 200 samples examined, 141 were positive by Giemsa staining, 146 by AO and 137 
      by QBC. Overall sensitivities for the 2 fluorescence techniques compared to 
      Giemsa staining were good: AO 97.9% and QBC 93.6%. However, with parasitaemias < 
      100/microL the QBC sensitivity fell to 41.7% whereas that of AO was 83.3%. Both 
      AO and QBC were unable to differentiate accurately between individual malaria 
      species. We conclude that the QBC technique alone cannot replace Giemsa-stained 
      thick blood films for most purposes in an African setting. However, apart from 
      species differentiation, the AO method is an appropriate technique for the 
      laboratory diagnosis of malaria in developing countries.
FAU - Lowe, B S
AU  - Lowe BS
AD  - Kenya Medical Research Institute, CRC Kilifi Unit, Kenya.
FAU - Jeffa, N K
AU  - Jeffa NK
FAU - New, L
AU  - New L
FAU - Pedersen, C
AU  - Pedersen C
FAU - Engbaek, K
AU  - Engbaek K
FAU - Marsh, K
AU  - Marsh K
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Trans R Soc Trop Med Hyg
JT  - Transactions of the Royal Society of Tropical Medicine and Hygiene
JID - 7506129
RN  - 0 (Azure Stains)
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
EIN - Trans R Soc Trop Med Hyg 1996 Mar-Apr;90(2):102
MH  - Acridine Orange
MH  - Adult
MH  - Animals
MH  - Azure Stains
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - Malaria/*diagnosis
MH  - Male
MH  - Plasmodium/isolation & purification
MH  - Sensitivity and Specificity
MH  - Staining and Labeling/*methods
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1016/s0035-9203(96)90470-8 [doi]
PST - ppublish
SO  - Trans R Soc Trop Med Hyg. 1996 Jan-Feb;90(1):34-6. doi: 
      10.1016/s0035-9203(96)90470-8.