PMID- 8730968
OWN - NLM
STAT- MEDLINE
DCOM- 19960924
LR  - 20220408
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 17
IP  - 8
DP  - 1996 Apr
TI  - In vitro biocompatibility of bioresorbable polymers: poly(L, DL-lactide) and 
      poly(L-lactide-co-glycolide).
PG  - 831-9
AB  - The acute toxicity of two degradable polymers, a 70:30 poly (L-D, L-lactide) 
      (PLDLA) and a 90:10 poly(L-lactide-co-glycolide) (PLGA), was evaluated by the 
      agar diffusion test and the filter test with L929 mouse fibroblasts. Extracts of 
      the materials prepared in phosphate-buffered saline at 37 and 70 degrees C were 
      assessed for mitochondrial succinate dehydrogenase activity by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and the 
      incorporation of 5-bromo-2'-deoxyuridine (BrdU) into DNA of BALB 3T3 cells. Both 
      materials revealed no signs of cytotoxicity during the agar diffusions and filter 
      tests. In the MTT and BrdU assays PLDLA and PLGA showed similar results. Cells 
      treated with extracts prepared at 37 degrees C caused slight stimulation of 
      mitochondrial activity. In contrast, cells incubated with the 70 degrees C media 
      revealed a concentration-dependent decrease of mitochondrial activity. DNA 
      synthesis was significantly decreased by the 37 degrees C extracts. As in the MTT 
      assay, the effect of the extracts prepared at 70 degrees C was significantly 
      greater. From these in vitro results it is suggested that PLDLA and PLGA have 
      satisfactory biocompatibility. High concentrations of the degradation products, 
      however, had a toxic influence on the cell culture systems used.
FAU - Ignatius, A A
AU  - Ignatius AA
AD  - Abteilung Unfallchirurgische Forschung und Biomechanik, Universitat Uim, Germany.
FAU - Claes, L E
AU  - Claes LE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Antimetabolites)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Buffers)
RN  - 0 (Coloring Agents)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polyesters)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 9007-49-2 (DNA)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EUY85H477I (thiazolyl blue)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - 3T3 Cells/cytology/drug effects
MH  - Animals
MH  - Antimetabolites/metabolism
MH  - *Biocompatible Materials
MH  - Bromodeoxyuridine/metabolism
MH  - Buffers
MH  - Cells, Cultured
MH  - Coloring Agents/chemistry
MH  - DNA/biosynthesis
MH  - Fibroblasts/cytology/drug effects/*metabolism
MH  - Fluorescent Dyes/chemistry
MH  - Immunodiffusion
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitochondria/drug effects/enzymology
MH  - Polyesters/chemistry/*metabolism/toxicity
MH  - Polyglycolic Acid/chemistry/*metabolism/toxicity
MH  - Succinate Dehydrogenase/metabolism
MH  - Tetrazolium Salts/chemistry
MH  - Thiazoles/chemistry
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 0142961296814219 [pii]
AID - 10.1016/0142-9612(96)81421-9 [doi]
PST - ppublish
SO  - Biomaterials. 1996 Apr;17(8):831-9. doi: 10.1016/0142-9612(96)81421-9.