PMID- 8731393
OWN - NLM
STAT- MEDLINE
DCOM- 19960926
LR  - 20220311
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 4
IP  - 1
DP  - 1996 Mar
TI  - Tumor necrosis factor-alpha (TNF-alpha) in canine osteoarthritis: 
      Immunolocalization of TNF-alpha, stromelysin and TNF receptors in canine 
      osteoarthritic cartilage.
PG  - 23-34
AB  - The presence and distribution of tumor necrosis factor-alpha (TNF-alpha), TNF 
      receptors and stromelysin [matrix metalloproteinase 3 (MMP-3)] in articular 
      cartilage were evaluated in an iatrogenically induced model of osteoarthritis 
      (OA). Eleven adult male dogs were assigned randomly to a control group (N = 4) or 
      an OA group (N = 7). Osteoarthritis was created by surgical transection of the 
      cranial cruciate ligament of one stifle joint. Both femoral condyles were sampled 
      3 months post-surgery at necropsy and immunohistochemically analyzed for the 
      presence of the aforementioned cytokines and receptors. Chondrocytes stained for 
      TNF-alpha and TNF receptors in control articular cartilage, spanning an area 
      encompassing most of the middle and deep zones. Positive matrical and 
      chondrocytic staining for TNF-alpha, TNF receptors, and stromelysin was present 
      in OA articular cartilage. Staining varied in intensity and distribution and was 
      dependent of the severity of the lesion. Smooth muscle cells of arteries and 
      arterioles (periarticular synovial membrane) were stained for only one (p55) of 
      two TNF receptors; this staining was confined to control tissues. Results 
      indicate that the differential expression of TNF-alpha and its receptors may be 
      important in the normal maintenance of articular cartilage. The increased 
      presence of TNF-alpha and its receptors in articular cartilage with mild 
      osteoarthritic changes suggests a role in the development of early OA. Regulating 
      TNF-alpha may be an important component in the treatment of OA.
FAU - Kammermann, J R
AU  - Kammermann JR
AD  - Department of Anatomy and Histology, College of Veterinary Medicine, Auburn 
      University, Alabama 36849-5518, USA.
FAU - Kincaid, S A
AU  - Kincaid SA
FAU - Rumph, P F
AU  - Rumph PF
FAU - Baird, D K
AU  - Baird DK
FAU - Visco, D M
AU  - Visco DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Disease Models, Animal
MH  - Dogs
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 3
MH  - Metalloendopeptidases/*metabolism
MH  - Muscle, Smooth, Vascular/metabolism
MH  - Osteoarthritis/*metabolism/pathology
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Synovial Membrane/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - S1063-4584(96)80004-5 [pii]
AID - 10.1016/s1063-4584(96)80004-5 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 1996 Mar;4(1):23-34. doi: 
      10.1016/s1063-4584(96)80004-5.