PMID- 8732501
OWN - NLM
STAT- MEDLINE
DCOM- 19961112
LR  - 20121115
IS  - 0022-2828 (Print)
IS  - 0022-2828 (Linking)
VI  - 28
IP  - 4
DP  - 1996 Apr
TI  - Adenovirus-mediated overexpression of human transforming growth factor-beta 1 in 
      rat cardiac fibroblasts, myocytes and smooth muscle cells.
PG  - 735-42
AB  - Transforming growth factor-beta 1 (TGF-beta 1) is known to regulate cardiac cell 
      function and its overexpression in the heart is thought to contribute to the 
      development of cardiac hypertrophy and fibrosis. We wished to develop a high 
      efficiency gene transfer method that could be used both in vitro and in vivo and 
      result in the overexpression of TGF-beta 1. For this purpose, we constructed a 
      replication-deficient human adenovirus 5 vector encoding for human TGF-beta 1 and 
      used for control purposes an adenovirus lacZ vector. The adenovirus 5 construct 
      was capable of infecting neonatal rat cardiac myocytes, fibroblasts and VSMCs. Of 
      the three cell types, cardiac myocytes appear more susceptible to infection by 
      the adenovirus 5 construct as assessed through beta-galactosidase staining. 
      Infection of cardiac fibroblasts, myocytes and VSMCs with the hTGF-beta 1 
      adenovirus leads to the expression of hTGF-beta 1 mRNA and enhanced levels of 
      bioactive and total TGF-beta 1 protein. Infection with hTGF-beta 1 adenovirus 
      also results in enhanced levels of collagen type III gene expression in VSMCs and 
      fibroblasts whereas in cardiac myocytes it leads to increased levels for 
      sarcomeric and beta-actin. Thus, this adenoviral vector might be used for the 
      exploration of in vivo effects of altered levels of cardiac TGF-beta 1.
FAU - Villarreal, F J
AU  - Villarreal FJ
AD  - Department of Medicine, University of California, San Diego 92103, USA.
FAU - Lee, A A
AU  - Lee AA
FAU - Dillmann, W H
AU  - Dillmann WH
FAU - Giordano, F J
AU  - Giordano FJ
LA  - eng
GR  - HL-43617/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Cell Cardiol
JT  - Journal of molecular and cellular cardiology
JID - 0262322
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Fibroblasts/metabolism
MH  - Fibrosis/genetics
MH  - *Gene Expression Regulation
MH  - Gene Transfer Techniques
MH  - Genetic Therapy
MH  - Genetic Vectors/chemistry
MH  - Humans
MH  - Hypertrophy/genetics
MH  - Muscle, Smooth, Vascular/cytology/metabolism
MH  - Myocardium/chemistry/cytology/*metabolism
MH  - Rats
MH  - Transforming Growth Factor beta/*genetics/*metabolism
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - S0022-2828(96)90068-1 [pii]
AID - 10.1006/jmcc.1996.0068 [doi]
PST - ppublish
SO  - J Mol Cell Cardiol. 1996 Apr;28(4):735-42. doi: 10.1006/jmcc.1996.0068.