PMID- 8735103
OWN - NLM
STAT- MEDLINE
DCOM- 19961210
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 34
IP  - 6
DP  - 1996 Jun
TI  - Antigenic variability among North American and European strains of porcine 
      reproductive and respiratory syndrome virus as defined by monoclonal antibodies 
      to the matrix protein.
PG  - 1488-93
AB  - Two hybridoma cell lines producing monoclonal antibodies (Mabs) to the 19-kDa 
      matrix (M) protein of porcine reproductive and respiratory syndrome virus (PRRSV) 
      were obtained from BALB/c mice that were immunized with a reference Quebec tissue 
      culture-adapted strain (strain IAF-Klop). The polypeptide specificities of the 
      MAbs were determined by immunoblotting and immunoprecipitation tests with 
      concentrated and purified preparations of the virus and by determining their 
      reactivities with the Escherichia coli-expressed gene products of open reading 
      frames 5 to 7. The two anti-M protein MAbs (MAbs IAFK3 and IAFK6) and another MAb 
      (MAb IAFK8) directed to the 15-kDa nucleocapsid (N) protein were devoid of 
      virus-neutralizing activity. A library of four anti-N protein MAbs (MAbs IAFK8, 
      SDOW17, VO17, and EP147) and two anti-M protein MAbs (MAbs IAFK6 and IAFK3) was 
      used to investigate, by an indirect fluorescent-antibody assay, the antigenic 
      diversity of 15 Canadian PRRSV isolates, in comparison with those of the U.S. 
      ATCC VR2332 attenuated vaccine strain and two reference European (Lelystad and 
      Weybridge) PRRSV strains. The North American and European PRRSV isolates tested 
      shared the epitopes recognized by anti-N protein MAbs IAFK8 and SDOW17, but three 
      distinct patterns could be identified on the basis of their reactivities with the 
      other anti-PRRSV MAbs. No reactivity to the anti-M protein MAbs was observed by 
      either European PRRSV isolate or the attenuated U.S. vaccine strain.
FAU - Dea, S
AU  - Dea S
AD  - Centre de Recherche en Virologie, Institut Armand-Frappier, Universite du Quebec, 
      Laval, Canada. Serge_Dea@IAF.UQUEBEC.CA
FAU - Gagnon, C A
AU  - Gagnon CA
FAU - Mardassi, H
AU  - Mardassi H
FAU - Milane, G
AU  - Milane G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - *Antigenic Variation
MH  - *Antigens, Viral
MH  - Cross Reactions
MH  - Europe
MH  - Female
MH  - Hybridomas/immunology
MH  - Mice
MH  - North America
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification
MH  - Swine
MH  - Viral Matrix Proteins/immunology
PMC - PMC229047
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
PMCR- 1996/06/01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PHST- 1996/06/01 00:00 [pmc-release]
AID - 10.1128/jcm.34.6.1488-1493.1996 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1996 Jun;34(6):1488-93. doi: 10.1128/jcm.34.6.1488-1493.1996.