PMID- 8735822
OWN - NLM
STAT- MEDLINE
DCOM- 19961216
LR  - 20190914
IS  - 0957-5235 (Print)
IS  - 0957-5235 (Linking)
VI  - 7
IP  - 2
DP  - 1996 Mar
TI  - Redistribution of membrane glycoproteins in platelets activated under flow 
      conditions.
PG  - 214-7
AB  - A reduction in the ability of GPIb to bind specific MoAbs or ligands (vWF) has 
      been reported in platelets exposed to thrombin in suspension. We have analyzed 
      modifications in the presence of glycoproteins (GPs) on platelets activated under 
      flow conditions in a system which allows limited thrombin and fibrin generation. 
      Normal blood anticoagulated with low molecular weight heparin (LMWH, Dalteparin 
      20 IU/ml) was recirculated for up to 10 min at 800 s-1 through annular chambers 
      containing denuded arterial segments. Aliquots of blood were removed from the 
      reservoir at 0, 1, 5 and 10 min and immediately mixed with paraformaldehyde. 
      Membrane glycoproteins: GPIb (CD42b), GPIIb-IIIa (CD41a), GPIV (CD36); and 
      activation dependent antigens: P-selectin (CD62P) and lysosomal glycoprortein 
      (CD63), were detected in whole blood by dual color flow cytometry. Circulation of 
      through the perfusion system resulted in platelet activated as demonstrated by 
      the increased percentage of platelets positive for antigens CD62P and CD63. A 
      gradual increase in the binding of MoAbs directed against GPIb, GPIIb-IIIa, and 
      GPIV epitopes was noted during the entire perfusion period. Observed differences 
      in mean fluorescence intensities at all the observation times were statistically 
      significant (P < 0.001). Our results obtained on platelets in an experimental 
      thrombosis system indicate that GPIb, GPIIb-IIIa and GPIV remain on the surface 
      of activated platelets and actually increase their expression. Alterations 
      detected at the level of GPIb in platelets activated by thrombin in suspension 
      may not take place under in vivo situations.
FAU - Lozano, M
AU  - Lozano M
AD  - Servicio de Hemoterapia y Hemostasia, Hospital Clinic, Facultat de Medicina, 
      Universitat de Barcelona, Spain.
FAU - Escolar, G
AU  - Escolar G
FAU - White, J G
AU  - White JG
FAU - Tassies, D
AU  - Tassies D
FAU - Ordinas, A
AU  - Ordinas A
FAU - Diaz-Ricart, M
AU  - Diaz-Ricart M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Blood Coagul Fibrinolysis
JT  - Blood coagulation & fibrinolysis : an international journal in haemostasis and 
      thrombosis
JID - 9102551
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD36 Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
RN  - 25422-31-5 (Fibrinopeptide A)
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Blood Platelets/*metabolism
MH  - CD36 Antigens/metabolism
MH  - Fibrin/metabolism
MH  - Fibrinopeptide A/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Membrane Glycoproteins/*metabolism
MH  - Platelet Activation
MH  - Platelet Glycoprotein GPIIb-IIIa Complex/metabolism
MH  - Platelet Glycoprotein GPIb-IX Complex/metabolism
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 10.1097/00001721-199603000-00026 [doi]
PST - ppublish
SO  - Blood Coagul Fibrinolysis. 1996 Mar;7(2):214-7. doi: 
      10.1097/00001721-199603000-00026.