PMID- 8738179
OWN - NLM
STAT- MEDLINE
DCOM- 19970218
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 41
IP  - 2
DP  - 1996 Apr
TI  - Prokaryotic expression of an immediate-early gene of human herpesvirus 6 and 
      analysis of its viral antigen expression in human cells.
PG  - 193-200
AB  - Segments of an immediate-early (1E) protein (1E03; 958 amino acids (aa)), encoded 
      by clone pSTY03, of human herpesvirus 6 (HHV-6) variant B strain HST were 
      expressed as beta-galactosidase fusion proteins in Escherichia coli. Using 
      Western blot analysis, and the serum of a patient having high titer anti-HHV-6 
      antibodies, an antigenic region of the IE03 protein was mapped between residues 
      340 and 505 (pUE03IE-M). The fusion protein expressed in E. coli harboring 
      plasmid pUE03IE-M was purified after electrophoresis in SDS-PAGE, and then 
      immunized in mice to obtain a monospecific antibody. Monospecific antibody raised 
      against the fusion protein reacted with IE03 protein species with apparent 
      molecular weights of 155 and 170 kDa, and was detected as granular fluorescence 
      in nuclei of infected cells by an immunofluorescence antibody test. Furthermore, 
      this antibody reacted only with HHV-6 variant B, but did not react with HHV-6 
      variant A. The IE03 protein was confirmed to be an IE protein, since the 
      synthesis of this protein was observed in infected cells that were first treated 
      with cycloheximide, which was then replaced with actinomycin D. Further, it was 
      also detected as early as 4 h after infection.
FAU - Takeda, K
AU  - Takeda K
AD  - Department of Virology, Osaka University, Japan.
FAU - Nakagawa, N
AU  - Nakagawa N
FAU - Yamamoto, T
AU  - Yamamoto T
FAU - Inagi, R
AU  - Inagi R
FAU - Kawanishi, K
AU  - Kawanishi K
FAU - Isegawa, Y
AU  - Isegawa Y
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (IE03 protein, herpes simplex virus 6)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (herpes simplex virus IE3 protein, Human herpesvirus 1)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antigens, Viral/*genetics/immunology
MH  - Cells, Cultured
MH  - Cycloheximide/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Exanthema Subitum/blood/immunology/virology
MH  - Fetal Blood/cytology
MH  - *Gene Expression
MH  - Herpesvirus 6, Human/genetics/immunology/*metabolism
MH  - Humans
MH  - Immediate-Early Proteins/*genetics/*immunology
MH  - Mice
MH  - Prokaryotic Cells/metabolism
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - *Viral Proteins
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 0168170296012877 [pii]
AID - 10.1016/0168-1702(96)01287-7 [doi]
PST - ppublish
SO  - Virus Res. 1996 Apr;41(2):193-200. doi: 10.1016/0168-1702(96)01287-7.