PMID- 8738182
OWN - NLM
STAT- MEDLINE
DCOM- 19961021
LR  - 20190815
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 14
IP  - 5
DP  - 1996 Mar
TI  - Half-fraction and full factorial designs versus central composite design for 
      retention modelling in reversed-phase ion-pair liquid chromatography.
PG  - 525-41
AB  - In a previous paper (J. O. De Beer, C. V. Vandenbroucke and D. L. Massart, J. 
      Pharm. Biomed. Anal., 12, (1994) 1379-1396) liquid chromatographic (LC) retention 
      modelling of the cough-syrup compounds methyl para-hydroxybenzoate (MPHB) and 
      propyl para-hydroxybenzoate (PPHB), phenylephrine hydrochloride (PE) and 
      chlorphenamine maleate (CPM) was studied using a face-centred central composite 
      design. It is examined whether smaller half-fractional and full factorial designs 
      with fewer experiments tend to reliably predict retention times of the latter 
      compounds as well. Simplified regression modelling, however, neglecting more 
      first-order and interactive effects and disregarding pure second-order effects, 
      has to be set up. These smaller designs finally satisfy the prediction of the 
      retention of MPHB, PPHB and PE also. Retention prediction of CPM is much less 
      accurate. CPM has a pKa value of 4.0, which is encompassed by the examined mobile 
      phase pH limits 3.0 and 5.0. Since the largest retention shifts occur near the 
      pKa value, retention prediction in this area becomes more complex. CPM retention 
      modelling from a full factorial design is useful if the mobile phase pH is fixed 
      at 5.0 for methanol as well as for acetonitrile as organic modifers. The full 
      factorial design, applied with acetonitrile as organic modifer, enables the 
      selection of suitable LC parameter combinations for fast and complete separation 
      of the four compounds in cough-syrup analysis.
FAU - De Beer, J O
AU  - De Beer JO
AD  - Institute for Hyginene and Epidemiology, Brussels, Belgium.
FAU - Vandenbroucke, C V
AU  - Vandenbroucke CV
FAU - Massart, D L
AU  - Massart DL
FAU - De Spiegeleer, B M
AU  - De Spiegeleer BM
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Parabens)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 3U6IO1965U (Chlorpheniramine)
RN  - A2I8C7HI9T (methylparaben)
RN  - Z8IX2SC1OH (propylparaben)
SB  - IM
MH  - Chemical Phenomena
MH  - Chemistry, Physical
MH  - Chlorpheniramine
MH  - Chromatography, Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Multivariate Analysis
MH  - Parabens
MH  - Phenylephrine
MH  - Predictive Value of Tests
MH  - *Regression Analysis
MH  - Statistics as Topic/*methods
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
AID - 0731708595016554 [pii]
AID - 10.1016/0731-7085(95)01655-4 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 1996 Mar;14(5):525-41. doi: 10.1016/0731-7085(95)01655-4.