PMID- 8739299
OWN - NLM
STAT- MEDLINE
DCOM- 19970114
LR  - 20131121
IS  - 0065-1281 (Print)
IS  - 0065-1281 (Linking)
VI  - 98
IP  - 2
DP  - 1996 Apr
TI  - Quantitative enzyme- and immunohistochemistry of hexokinase and cytochrome c 
      oxidase of spinal neurons in the zebrafish.
PG  - 143-55
AB  - A combined quantitative enzyme- and immunohistochemical procedure to demonstrate 
      hexokinase (HK) was developed and tested on sections of spinal cord tissue of the 
      zebrafish. In both procedures, the amount of final reaction product was linearly 
      related with section thickness. When applied to serial sections of fish spinal 
      neurons, the enzyme- and immunohistochemical activities appeared to correlate 
      significantly (r = 0.61; p < 0.001). As HK and cytochrome c oxidase (COX) 
      histochemistry have been used regularly to screen the average level of chronic 
      activity of neurons, we subsequently analysed the relationship between HK and COX 
      in fish spinal neurons, using previously published methods of quantitative 
      enzyme- and immunohistochemistry for COX. The enzyme- as well as the 
      immunohistochemical localisation patterns of HK showed a weak correlation with 
      the enzyme- and immunohistochemical COX localisation respectively. Therefore, it 
      is concluded that both enzyme- and immunohistochemical localisation of COX 
      provide a poor estimate for the relative level of glucose utilisation in fish 
      spinal neurons.
FAU - van Raamsdonk, W
AU  - van Raamsdonk W
AD  - Department of Experimental Zoology, University of Amsterdam, The Netherlands.
FAU - Smit-Onel, M J
AU  - Smit-Onel MJ
FAU - Diegenbach, P C
AU  - Diegenbach PC
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Antibodies)
RN  - 53-59-8 (NADP)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - I38ZP9992A (Magnesium)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenosine Triphosphate
MH  - Animals
MH  - Antibodies/immunology
MH  - Electron Transport Complex IV/*analysis/immunology
MH  - Glucose/metabolism
MH  - Hexokinase/*analysis/immunology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Magnesium
MH  - NADP
MH  - Neurons/*enzymology
MH  - Spinal Cord/cytology/*enzymology
MH  - Zebrafish
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - S0065-1281(96)80032-8 [pii]
AID - 10.1016/S0065-1281(96)80032-8 [doi]
PST - ppublish
SO  - Acta Histochem. 1996 Apr;98(2):143-55. doi: 10.1016/S0065-1281(96)80032-8.