PMID- 8741275
OWN - NLM
STAT- MEDLINE
DCOM- 19970221
LR  - 20190516
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 58
IP  - 4
DP  - 1996 Apr
TI  - Characteristics of major structural protein coding gene and leader-body sequence 
      in subgenomic mRNA of porcine reproductive and respiratory syndrome virus 
      isolated in Japan.
PG  - 377-80
AB  - Nucleotide sequence, 1713nt in length, of porcine reproductive and respiratory 
      syndrome virus (PRRSV) isolated in Japan was determined. The sequence encompassed 
      3 overlapping open reading frames (ORFs), ORF5 to ORF7. These ORFs encodes major 
      structural proteins of PRRSV. The deduced amino acid sequence of each ORF showed 
      higher than 87.5% identity with an American isolate, and lower (54.6 to 80.5%) 
      identity with an European isolate. This result supported a previous report about 
      antigenic characteristics of the EDRD-1 strain. Leader-body junction sequence in 
      subgenomic mRNA of the EDRD-1 strain was determined by sequencing cDNA clones of 
      subgenomic RNAs. A common sequence motif of 5 nucleotide, represented by 
      UA(A/G)CC, was identified as the junction sequence.
FAU - Saito, A
AU  - Saito A
AD  - National Veterinary Assay Laboratory, Tokyo, Japan.
FAU - Kanno, T
AU  - Kanno T
FAU - Murakami, Y
AU  - Murakami Y
FAU - Muramatsu, M
AU  - Muramatsu M
FAU - Yamaguchi, S
AU  - Yamaguchi S
LA  - eng
SI  - GENBANK/D45852
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA Primers)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Messenger)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arterivirus/*genetics/isolation & purification
MH  - Arterivirus Infections/*veterinary/virology
MH  - Base Sequence
MH  - DNA Primers
MH  - Japan
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Protein Sorting Signals/genetics
MH  - RNA, Messenger/analysis
MH  - Swine
MH  - *Swine Diseases
MH  - Viral Structural Proteins/*genetics
EDAT- 1996/04/01 00:00
MHDA- 1996/04/01 00:01
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 1996/04/01 00:01 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1292/jvms.58.377 [doi]
PST - ppublish
SO  - J Vet Med Sci. 1996 Apr;58(4):377-80. doi: 10.1292/jvms.58.377.