PMID- 8741576
OWN - NLM
STAT- MEDLINE
DCOM- 19970102
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 19
IP  - 5
DP  - 1996 May
TI  - Effects of methyl 9(or 10)-hydroxy-10(or 9)-mercaptostearate and hexadecanethioic 
      S-acid on cupric ion- or 2,2-azo-bis(2-amidinopropane) dihydrochloride 
      (AAPH)-induced oxidation of low density lipoprotein.
PG  - 692-6
AB  - The preventive effects of two antioxidants, methyl 9(or 10)-hydroxy-10 (or 
      9)-mercaptostearate (SH-S) and hexadecanethioic S-acid (thiopalmitic acid, 
      SH-Pal) against the oxidative modification of low density lipoproteins (LDL) 
      induced by cupric ion or a water soluble initiator of peroxyl radicals, 
      2,2-azobis(2-amidinopropane) dihydrochloride (AAPH), were studied by measuring 
      thiobarbituric acid-reactive substances (TBARS). SH-S acted as an effective 
      antioxidant in the oxidative modification of LDL induced by either cupric ion or 
      AAPH. Interestingly, SH-S completely inhibited the formation of fluorescence 
      products and decreased both the fluorescence and alpha-tocopherol content in LDL 
      induced by cupric ion, and reduced 1,1-diphenyl 2-picrylhydrazyl (DPPH) used as a 
      stable free radical model. The antioxidative effect was effectively prevented by 
      the addition of increasing amounts of N-ethylmalemide (NEM) to the system. SH-Pal 
      also inhibited the cupric ion-induced LDL oxidation, but showed little inhibitory 
      effect on the AAPH-induced LDL oxidation. Moreover, SH-Pal was reduced to 
      palmitic acid during the AAPH-induced LDL oxidation. These findings indicate that 
      SH-S protects against oxidative damage of LDL in vitro, and that it acts as a 
      free radical in peroxidation. In addition, this study shows that SH-Pal doesn't 
      act as an efficient antioxidant in AAPH-induced lipid peroxidation.
FAU - Tanaka, M
AU  - Tanaka M
AD  - Showa College of Pharmaceutical Sciences, Tokyo, Japan.
FAU - Takada, K
AU  - Takada K
FAU - Higuchi, T
AU  - Higuchi T
FAU - Nakagawa, M
AU  - Nakagawa M
FAU - Murase, M
AU  - Murase M
FAU - Tobinaga, S
AU  - Tobinaga S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Amidines)
RN  - 0 (Antioxidants)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Palmitic Acids)
RN  - 0 (Stearates)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (methyl-9-hydroxy-10-mercaptostearate)
RN  - 0 (thiopalmitic acid)
RN  - 1406-18-4 (Vitamin E)
RN  - 7381JDR72F (2,2'-azobis(2-amidinopropane))
RN  - LRX7AJ16DT (Copper Sulfate)
SB  - IM
MH  - Amidines
MH  - Antioxidants/*pharmacology
MH  - Copper Sulfate
MH  - Fluorescence
MH  - Humans
MH  - In Vitro Techniques
MH  - Lipid Peroxidation/*drug effects
MH  - Lipoproteins, LDL/blood/*chemistry
MH  - Palmitic Acids/*pharmacology
MH  - Stearates/*pharmacology
MH  - *Sulfhydryl Compounds
MH  - Thiobarbituric Acid Reactive Substances
MH  - Vitamin E/analysis/chemistry
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1248/bpb.19.692 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1996 May;19(5):692-6. doi: 10.1248/bpb.19.692.