PMID- 8741581
OWN - NLM
STAT- MEDLINE
DCOM- 19970102
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 19
IP  - 5
DP  - 1996 May
TI  - Lipid peroxidation in gastric mucosal lesions induced by indomethacin in rat.
PG  - 716-20
AB  - The peroxidation of lipids and changes in the activities of related enzymes in 
      the gastric mucosa were studied in a rat model of gastric mucosal injury induced 
      by the nonsteroidal anti-inflammatory drug indomethacin. The area of gastric 
      erosion and the amount of thiobarbituric acid reactive substances (TBARS) in 
      gastric mucosa were significantly increased beginning 4h after administration of 
      indomethacin. Xanthine oxidase (XOD) activity in the gastric mucosa also 
      increased immediately after administration of the drug. Although XOD activity was 
      significantly suppressed by allopurinol treatment, the induction of gastric 
      mucosal injury and the increase of TBARS in the gastric mucosa were not. 
      Myeloperoxidase (MPO), a marker enzyme of leukocytes, was unaffected by 
      indomethacin administration. But the depletion of polymorphonuclear leukocyte 
      (PMN) counts induced by an injection of anti-rat PMN antibody inhibited both the 
      injury and the increase in TBARS. Indomethacin activated PMN in peripheral blood 
      at 30mg/kg per as and enhanced release of oxygen radicals from PMN in peripheral 
      blood. As compared with the XOD system, the generation of oxygen free radicals 
      may derived mainly from activated PMN. On the other hand, superoxide dismutase 
      (SOD) and glutathione peroxidase (GSH-px) were reduced by the administration of 
      indomethacin. Decreases in SOD and GSH-px activity in gastric mucosa may 
      aggravate mucosal injury by free radicals and lipid peroxidation.
FAU - Tanaka, J
AU  - Tanaka J
AD  - Pharmacology Dept., Meiji Seika Kaisha, Ltd., Yokohama, Japan.
FAU - Yuda, Y
AU  - Yuda Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antibodies)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 11062-77-4 (Superoxides)
RN  - 63CZ7GJN5I (Allopurinol)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.17.3.2 (Xanthine Oxidase)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Allopurinol/pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology/*toxicity
MH  - Antibodies/pharmacology
MH  - Gastric Mucosa/*drug effects/enzymology/metabolism
MH  - Indomethacin/pharmacology/*toxicity
MH  - *Lipid Peroxidation
MH  - Male
MH  - Neutrophils/drug effects/enzymology
MH  - Peroxidase/metabolism
MH  - Rats
MH  - Superoxide Dismutase/metabolism
MH  - Superoxides/metabolism
MH  - Thiobarbituric Acid Reactive Substances/analysis
MH  - Xanthine Oxidase/metabolism
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
AID - 10.1248/bpb.19.716 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1996 May;19(5):716-20. doi: 10.1248/bpb.19.716.