PMID- 8742189
OWN - NLM
STAT- MEDLINE
DCOM- 19961011
LR  - 20131121
IS  - 0004-1955 (Print)
IS  - 0004-1955 (Linking)
VI  - 57
IP  - 6
DP  - 1995 Nov-Dec
TI  - [Enzymic spectrum of preneoplastic and neoplastic changes induced by 
      1,2-dimethylhydrazine in mouse kidneys].
PG  - 52-7
AB  - Mouse renal cell tumors (RCT) were induced in male CBA male mice by 5 
      subcutaneous injections of 8 mg 1,2-dimethylhydrazine (DMH) per kg body weight 
      once a week. After a lag period of two years the kidneys were removed, and serial 
      cryostat sections of the kidneys were histochemically analyzed for the following 
      parameters: Glycogen content, basophilia, and activities of glycogen synthase 
      (SYN), glycogen phosphorylase (PHO), glucose-6-phosphatase (G6Pase), 
      glucose-6-phosphate dehydrogenase (G6PDH), hexokinase (HK), pyruvate kinase (PK), 
      lactate dehydrogenase (LDH), malic enzyme (ME), succinate dehydrogenase (SDH), 
      alkaline phosphatase (ALPase) and glutamyl-transpeptidase (GGT). RCT displayed 
      the same histochemical profile irrespective of their size and growth pattern. In 
      comparison with normal kidney epithelium, the neoplastic cells exhibited elevated 
      activities of enzymes for glycolysis (HK, PK LDH) and the pentose phosphate 
      pathway (G6PDH) while negative G6Pase and low SDH activity were observed in these 
      cells. The majority of RCT showed high PHO activity and weak staining for SYN. 
      Activities of ALPase and GGT were negative in most of the RCT. Giant cells were 
      detected in some large RCT. Higher activities of glycolytic and mitochondrial 
      enzymes and G6PDH were found in giant cells compared with other tumor cells. 
      Tubular preneoplastic lesions were similar to neoplastic lesions in morphological 
      and histochemical characteristics. The present study revealed that a markedly 
      elevated capacity for glycolysis and the pentose phosphate pathway occurred in 
      renal cell tumors in mice. A similar histochemical pattern in the few 
      preneoplastic tubular lesions observed suggests that these metabolic aberrations 
      emerge early in carcinogenesis, but studies on earlier stages of renal 
      carcinogenesis are needed to substantiate this assumption.
FAU - Ahn, I S
AU  - Ahn IS
AD  - Deutsches Krebsforschungszentrum, Heidelberg, Germany.
FAU - Cheteris, G Iu
AU  - Cheteris GIu
FAU - Turusov, V S
AU  - Turusov VS
FAU - Bannash, P
AU  - Bannash P
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Fermentny i spektr preneoplasticheskikh i neoplasticheksikh izmeneni i 
      vyzyvaemykh 1,2-dimetilgidrazinom v pochkakh myshe i.
PL  - Russia (Federation)
TA  - Arkh Patol
JT  - Arkhiv patologii
JID - 0370604
RN  - 0 (Carcinogens)
RN  - 0 (Dimethylhydrazines)
RN  - IX068S9745 (1,2-Dimethylhydrazine)
SB  - IM
MH  - 1,2-Dimethylhydrazine
MH  - Animals
MH  - Carcinogens
MH  - Carcinoma, Renal Cell/chemically induced/*enzymology
MH  - Dimethylhydrazines
MH  - Glycolysis/physiology
MH  - Kidney Neoplasms/chemically induced/*enzymology
MH  - Male
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Pentose Phosphate Pathway
MH  - Precancerous Conditions/chemically induced/*enzymology
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PST - ppublish
SO  - Arkh Patol. 1995 Nov-Dec;57(6):52-7.