PMID- 8743550
OWN - NLM
STAT- MEDLINE
DCOM- 19961120
LR  - 20131121
IS  - 0098-6577 (Print)
IS  - 0098-6577 (Linking)
VI  - 55
DP  - 1996 Jun
TI  - Functional analysis and chromosomal gene assignment of rat kidney prostaglandin 
      EP3 receptor.
PG  - S183-6
AB  - We have reported two isoformes of rat prostaglandin EP3 receptor with their 
      different carboxyl-terminal tails (rEP3A and rEP3B receptors), which are derived 
      by alternative RNA splicing, and both receptors have been shown to be localized 
      to renal distal tubules. In the present study, we characterized the signal 
      transduction system of rat kidney EP3 receptors either in a renal cell line 
      mimicking renal distal tubule cells, TKC2, or in COS-7 cells by functional 
      expression of these receptors. We also examined the chromosomal localization of 
      the EP3 receptor gene by fluorescence in situ hybridization (FISH). In TKC2 
      cells, vasopressin (AVP, 10(-7) M), prostaglandin (PG) E2 (10(-7) M), or 
      forskolin (10(-8) M) markedly stimulated cyclic AMP formation. Overexpression of 
      the rEP3A receptor significantly attenuated the AVP-, PGE2- or forskolin-induced 
      cyclic AMP formation, whereas there was no change with rEP3B receptor expression. 
      On the other hand, in COS-7 cells transfected with rEP3A receptor cDNA, PGE2 
      (10(-7) M) did not affect cytosolic free calcium concentration ([Ca2+]i), whereas 
      transfection of rEP3B receptor cDNA evoked PGE2-induced increases in [Ca2+]i. 
      Moreover, we have revealed that the rEP3 receptor gene is localized to rat 
      chromosome 2q44-45. In conclusion, rEP3A or rEP3B receptor is suggested as a 
      mediator of the natriuretic/diuretic action of PGE2 in renal distal tubules via a 
      decrease in cyclic AMP formation or an increase in [Ca2+]i, respectively. 
      Information of the gene assignment of rat EP3 receptor to rat chromosome 2q44-45 
      is useful for further analysis of the role of EP3 receptor in genetically 
      hypertensive rat models.
FAU - Takeuchi, K
AU  - Takeuchi K
AD  - Second Department of Internal Medicine, Tohoku University School of Medicine, 
      Sendai, Japan.
FAU - Takahashi, N
AU  - Takahashi N
FAU - Kato, T
AU  - Kato T
FAU - Abe, T
AU  - Abe T
FAU - Taniyama, Y
AU  - Taniyama Y
FAU - Tsutsumi, E
AU  - Tsutsumi E
FAU - Ito, O
AU  - Ito O
FAU - Nakagawara, K
AU  - Nakagawara K
FAU - Abe, K
AU  - Abe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int Suppl
JT  - Kidney international. Supplement
JID - 7508622
RN  - 0 (Receptors, Prostaglandin E)
RN  - 802-31-3 (prostaglandin E3)
RN  - E0399OZS9N (Cyclic AMP)
RN  - F5TD010360 (Alprostadil)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alprostadil/*analogs & derivatives/metabolism
MH  - Animals
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Chromosome Mapping
MH  - Cyclic AMP/metabolism
MH  - In Situ Hybridization
MH  - Kidney/*metabolism/ultrastructure
MH  - Mice
MH  - Mice, Transgenic
MH  - Rats
MH  - Receptors, Prostaglandin E/*genetics/metabolism
MH  - Second Messenger Systems/physiology
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PST - ppublish
SO  - Kidney Int Suppl. 1996 Jun;55:S183-6.