PMID- 8745401
OWN - NLM
STAT- MEDLINE
DCOM- 19961017
LR  - 20181113
IS  - 0961-8368 (Print)
IS  - 1469-896X (Electronic)
IS  - 0961-8368 (Linking)
VI  - 5
IP  - 2
DP  - 1996 Feb
TI  - Structure-based modeling of the ligand binding domain of the human cell surface 
      receptor CD23 and comparison of two independently derived molecular models.
PG  - 240-7
AB  - CD23, a type II membrane receptor protein, recognizes four different ligands via 
      its extracellular C-type lectin domain: immunoglobulin E (IgE), CD21, and the 
      beta 2-integrins CD11b and CD11c. CD23 specifically interacts in a 
      calcium-dependent manner, "lectin-like" with carbohydrate moieties expressed on 
      CD21 and CD11b/c, but also "lectin-unlike" with protein epitopes on IgE. As a 
      first step in analyzing the multiple binding specificities associated with CD23 
      in more detail, we report a detailed molecular model of the lectin-like domain of 
      human CD23 (hCD23). The model was built based on information provided by X-ray 
      structures of mannose binding protein (MBP) and E-selectin, both of which are 
      members of the calcium-dependent (C-type) lectin superfamily. Sequence-structure 
      comparisons suggest that hCD23 is structurally more similar to MBP than to 
      E-selectin. The hCD23 model is compared to an independently derived model. 
      Although the CD23-carbohydrate and CD23-protein interactions are both calcium 
      dependent, analysis of the model suggests the presence of distinct binding sites 
      for these ligands.
FAU - Bajorath, J
AU  - Bajorath J
AD  - Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington 
      98121, USA.
FAU - Aruffo, A
AU  - Aruffo A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Protein Sci
JT  - Protein science : a publication of the Protein Society
JID - 9211750
RN  - 0 (E-Selectin)
RN  - 0 (Integrin alphaXbeta2)
RN  - 0 (Ligands)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Receptors, Complement 3d)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - Crystallography, X-Ray
MH  - E-Selectin/chemistry
MH  - Humans
MH  - Immunoglobulin E/metabolism
MH  - Integrin alphaXbeta2/metabolism
MH  - Ligands
MH  - Macrophage-1 Antigen/metabolism
MH  - *Models, Molecular
MH  - Molecular Sequence Data
MH  - Myelin Basic Protein/chemistry
MH  - Protein Binding
MH  - *Protein Conformation
MH  - Receptors, Complement 3d/metabolism
MH  - Receptors, IgE/*chemistry/metabolism
MH  - Structure-Activity Relationship
PMC - PMC2143337
EDAT- 1996/02/01 00:00
MHDA- 1996/02/01 00:01
PMCR- 1996/08/01
CRDT- 1996/02/01 00:00
PHST- 1996/02/01 00:00 [pubmed]
PHST- 1996/02/01 00:01 [medline]
PHST- 1996/02/01 00:00 [entrez]
PHST- 1996/08/01 00:00 [pmc-release]
AID - 10.1002/pro.5560050207 [doi]
PST - ppublish
SO  - Protein Sci. 1996 Feb;5(2):240-7. doi: 10.1002/pro.5560050207.