PMID- 8746510
OWN - NLM
STAT- MEDLINE
DCOM- 19960918
LR  - 20131121
IS  - 0021-2938 (Print)
IS  - 0021-2938 (Linking)
VI  - 44
IP  - 5
DP  - 1995 Sep-Oct
TI  - HPLC analysis of cyclic adenosine diphosphate ribose and adenosine diphosphate 
      ribose: determination of NAD+ metabolites in hippocampal membranes.
PG  - 258-68
AB  - Cyclic adenosine diphosphate-ribose (cADPR) and ADPR were separated by 
      high-performance liquid chromatography (HPLC) on a CarboPac PA-1 column at strong 
      basic pH and quantitated by a pulsed amperometric detector. Although this HPLC 
      method was quite sensitive and highly reproducible, it did not allow the 
      separation of cADPR from guanosine monophosphate (GMP) which, when present, could 
      be removed by ion-affinity chromatography, using gel-immobilized Fe3+ columns. 
      Crude synaptic membranes from rat hippocampi were incubated with nicotinamide 
      adenine dinucleotide (NAD) and acidic extracts were subject to HPLC analysis 
      after neutralization. Incubation led to a time-dependent formation of ADPR, which 
      was amplified when membranes were incubated in the presence of guanosine 
      trisphosphate (GTP), guanosine-5'-0-(3-thiotrisphosphate) (GTP-gamma-S) or AlF3. 
      cADPR did not accumulate in detectable amounts and only a minimal proportion (< 
      5%) of radioactivity originating from [3H]NAD co-eluted with authentic cADPR in 
      extracts from hippocampal membranes. The simultaneous detection of cADPR and ADPR 
      we have described may help the search for inhibitors of cADPR metabolism, which 
      will allow to measure the cADPR that accumulates under basal conditions or in 
      response to extracellular signals.
FAU - Casabona, G
AU  - Casabona G
AD  - Istituto di Farmacologia, Facolta di Medicina, Universita di Catania.
FAU - Sturiale, L
AU  - Sturiale L
FAU - L'Episcopo, M R
AU  - L'Episcopo MR
FAU - Raciti, G
AU  - Raciti G
FAU - Fazzio, A
AU  - Fazzio A
FAU - Sarpietro, M G
AU  - Sarpietro MG
FAU - Genazzani, A A
AU  - Genazzani AA
FAU - Cambria, A
AU  - Cambria A
FAU - Nicoletti, F
AU  - Nicoletti F
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Ital J Biochem
JT  - The Italian journal of biochemistry
JID - 0376564
RN  - 0U46U6E8UK (NAD)
RN  - 119340-53-3 (Cyclic ADP-Ribose)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
SB  - IM
MH  - Adenosine Diphosphate Ribose/*analogs & derivatives/*analysis
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Cyclic ADP-Ribose
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Membranes/metabolism
MH  - NAD/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1995/09/01 00:00
MHDA- 1995/09/01 00:01
CRDT- 1995/09/01 00:00
PHST- 1995/09/01 00:00 [pubmed]
PHST- 1995/09/01 00:01 [medline]
PHST- 1995/09/01 00:00 [entrez]
PST - ppublish
SO  - Ital J Biochem. 1995 Sep-Oct;44(5):258-68.