PMID- 8747992
OWN - NLM
STAT- MEDLINE
DCOM- 19961009
LR  - 20071115
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 13 Suppl 3
DP  - 1995 Dec
TI  - Diagnosis and monitoring of chromosome aberrations in hematological malignancies 
      by fluorescence in situ hybridization.
PG  - 76-82
AB  - Besides its application in biological research, fluorescence in situ 
      hybridization (FISH) is increasingly used for the cytogenetic analysis of human 
      malignancies. Compared to conventional cytogenetic analysis, FISH allows 
      delineation of specific numerical and structural chromosome aberrations in 
      interphase cells (interphase cytogenetics). We have developed sets of genomic DNA 
      probes for the identification of chromosome aberrations associated with chronic 
      lymphocytic leukemia (CLL), chronic myeloid leukemias (CML), and acute myeloid 
      leukemia (AML). In CLL, interphase cytogenetics will greatly contribute to the 
      evaluation of the true incidence of specific chromosome aberrations and will 
      provide the basis for more accurate correlations with the clinical outcome. The 
      Philadelphia chromosome can be detected by FISH with high specificity and 
      sensitivity in both CML and acute lymphoblastic leukemia. In CML, it can be used 
      to better assess the cytogenetic remission status following therapy with 
      interferon-alpha. Finally, in AML interphase cytogenetics provides a rapid and 
      reliable technique for the identification of chromosome aberrations which are one 
      of the most important prognostic factors in this disease. With the design of 
      complex DNA probe sets and the development of digital microscopy and automated 
      image analysis, it will be possible to use such disease-specific probe sets for 
      monitoring residual disease following chemotherapy.
FAU - Dohner, H
AU  - Dohner H
AD  - Medizinische Klinik und Poliklinik V, University of Heidelberg, Germany.
FAU - Stilgenbauer, S
AU  - Stilgenbauer S
FAU - Fischer, K
AU  - Fischer K
FAU - Schroder, M
AU  - Schroder M
FAU - Bentz, M
AU  - Bentz M
FAU - Lichter, P
AU  - Lichter P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Acute Disease
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/genetics
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/genetics
MH  - Leukemia, Myeloid/*diagnosis/genetics
RF  - 28
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - 10.1002/stem.5530130712 [doi]
PST - ppublish
SO  - Stem Cells. 1995 Dec;13 Suppl 3:76-82. doi: 10.1002/stem.5530130712.