PMID- 8751555
OWN - NLM
STAT- MEDLINE
DCOM- 19960918
LR  - 20061115
IS  - 0090-8258 (Print)
IS  - 0090-8258 (Linking)
VI  - 62
IP  - 2
DP  - 1996 Aug
TI  - CUMC-6, a new diploid human cell line derived from a squamous carcinoma of the 
      uterine cervix.
PG  - 230-40
AB  - A new cell line, CUMC-6, has been derived from an invasive nonkeratinizing 
      squamous cell carcinoma of the uterine cervix in a 31-year-old patient. It has 
      been maintained in long-term culture for 61 months, and passaged over 300 times. 
      Monolayer-cultured cells were polygonal in shape, showing a pavement-like 
      arrangement and a tendency to pile up without contact inhibition. The epithelial 
      nature of the cultured CUMC-6 cells was confirmed by transmission electron 
      microscopy which demonstrated the presence of desmosomes and tonofilaments. The 
      subcutaneous injection of cultured cells into nude mice gave rise to fast-growing 
      tumors. The transplanted tumor showed similar histological features, but poor 
      differentiation compared to the original tumor. Cultured CUMC-6 cells produced 
      human chorionic gonadotropin beta-subunit (beta-HCG) and alpha-fetoprotein (AFP). 
      Cytosol estrogen and progesterone receptors were not measured in this cell line. 
      The results of isozyme analyses were distinct from the HeLa cell line. Repeated 
      chromosome analysis from passage 6 to 300 revealed that most metaphases of this 
      cell line contained diploid number of chromosomes. The structural abnormality 
      consistently observed in this cell line was the elongation of short arm of 
      chromosome 1. The G- or R-banded pattern of this chromosome suggested inv dup (1) 
      (1pter-->1p34[symbol: see text] 1p21-->1p34[symbol: see text] 1p34-->1qter). 
      Human leukocyte antigen (HLA) typing of CUMC-6 cells indicated the presence of 
      DR12 and DQw3. Analysis of the DNA extracted from the CUMC-6 cells showed the 
      presence of human papillomavirus (HPV) type 16 and 18 DNAs. The results of 
      oncogene analyses using Southern blotting technique revealed amplification and 
      rearrangement of oncogene c-myc and no amplification of oncogene L-myc. Using the 
      polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) 
      technique, we have screened CUMC-6 cells for p53 mutation in exons 4 to 9. No 
      mobility shift was observed in this cell line. These results suggest that 
      chromosome 1 abnormality, oncogene alteration, and HPV infection work together in 
      cervical tumorigenesis.
FAU - Kim, J W
AU  - Kim JW
FAU - Lee, C G
AU  - Lee CG
FAU - Cho, Y H
AU  - Cho YH
FAU - Kim, J H
AU  - Kim JH
FAU - Kim, S J
AU  - Kim SJ
FAU - Kim, H K
AU  - Kim HK
FAU - Park, T C
AU  - Park TC
FAU - Song, S K
AU  - Song SK
FAU - Namkoong, S E
AU  - Namkoong SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gynecol Oncol
JT  - Gynecologic oncology
JID - 0365304
RN  - 0 (DNA Probes)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - DNA Probes
MH  - *Diploidy
MH  - Female
MH  - Genes, myc/genetics
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Receptors, Estrogen/analysis
MH  - Receptors, Progesterone/analysis
MH  - *Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/genetics/*pathology
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - S0090-8258(96)90221-8 [pii]
AID - 10.1006/gyno.1996.0221 [doi]
PST - ppublish
SO  - Gynecol Oncol. 1996 Aug;62(2):230-40. doi: 10.1006/gyno.1996.0221.