PMID- 8752732
OWN - NLM
STAT- MEDLINE
DCOM- 19961206
LR  - 20061115
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 44
IP  - 6
DP  - 1996 Jun
TI  - [Chromosome analysis and FISH method].
PG  - 541-7
AB  - In hematologic malignancies activation of oncogenes or inactivation of tumor 
      suppressor genes are thought to be responsible for its carcinogenesis, just like 
      other solid tumors. As these genetic changes are detected as chromosome 
      abnormalities, cytogenetic analysis has been used as a tool for diagnosis and 
      follow up after therapy. The fluorescence in situ hybridization (FISH) method is 
      a new technic which enables us to detect genetic changes both in mitotic and 
      interphase cells. It is a useful method in the clinical field which covers the 
      disadvantages in cytogenetic analysis, RT-PCR and Southern blotting. We 
      practically used the FISH method in diagnosis and follow-up observation of 
      minimal residual disease in several kinds of hematologic malignancies. By the 
      morphology-FISH method, the relationship between cell morphology and genetic 
      changes could be analyzed simultaneously. The FISH method was also applied to 
      probe mapping to identify a novel breakpoint cluster region in the 11q23 area in 
      adult hematological disease. FISH method is a rapid and powerful tool both in 
      clinical and basic study in hematologic disorders.
FAU - Eguchi, M
AU  - Eguchi M
AD  - Department of Cancer Cytogenetics, Hiroshima University.
FAU - Tanaka, K
AU  - Tanaka K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosome Disorders
MH  - *Chromosome Mapping
MH  - Hematologic Diseases/*diagnosis/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Neoplasm, Residual/diagnosis
RF  - 5
EDAT- 1996/06/01 00:00
MHDA- 1996/06/01 00:01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 1996/06/01 00:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 1996 Jun;44(6):541-7.