PMID- 8753795
OWN - NLM
STAT- MEDLINE
DCOM- 19961010
LR  - 20121115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 225
IP  - 2
DP  - 1996 Aug 14
TI  - Prevention of the expression of inducible nitric oxide synthase by aminoguanidine 
      or aminoethyl-isothiourea in macrophages and in the rat.
PG  - 525-30
AB  - An enhanced formation of nitric oxide (NO) following the induction of the 
      inducible isoform of NOS (iNOS) has been implicated in the pathogenesis of 
      circulatory shock and inflammation. This study elucidates the effects of the NOS 
      inhibitors aminoethyl-isothiourea (AE-ITU), aminoguanidine (AG), 
      NG-methyl-L-arginine (L-NMMA) or N omega-Nitro-L-arginine methyl ester (L-NAME) 
      on the expression of iNOS protein lipopolysaccharide (LPS) in macrophages and the 
      rat (lung homogenates). The expression of iNOS protein was detected by Western 
      blot analysis using a specific iNOS antibody. In the absence of LPS, the iNOS 
      protein was expressed neither in macrophages nor in the lung. LPS (1 
      microgram.ml-1) resulted in the expression of iNOS protein in macrophages, which 
      was significantly inhibited by pretreatment of cells with AE-ITU or AG, but not 
      by L-NMMA or L-NAME. In addition, LPS (10 mg.kg-1, i.v.) also caused an increase 
      in the expression of iNOS protein in lungs obtained from rats at 6 h after LPS, 
      which was significantly reduced by treatment of LPS-rats with AE-ITU or AG, but 
      not with L-NMMA or L-NAME. Thus, AE-ITU or AG inhibit not only iNOS activity, but 
      also the induction of iNOS protein in vitro and in vivo caused by endotoxin.
FAU - Ruetten, H
AU  - Ruetten H
AD  - William Harvey Research Institute, St. Bartholomew's Hospital Medical College, 
      London, United Kingdom.
FAU - Thiemermann, C
AU  - Thiemermann C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Guanidines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - SCQ4EZQ113 (pimagedine)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Enzyme Inhibitors/*pharmacology
MH  - Guanidines/*pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Lung/*drug effects/metabolism
MH  - Macrophages/*drug effects/enzymology
MH  - Male
MH  - Mice
MH  - Nitric Oxide Synthase/*antagonists & inhibitors/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1996/08/14 00:00
MHDA- 1996/08/14 00:01
CRDT- 1996/08/14 00:00
PHST- 1996/08/14 00:00 [pubmed]
PHST- 1996/08/14 00:01 [medline]
PHST- 1996/08/14 00:00 [entrez]
AID - S0006-291X(96)91206-1 [pii]
AID - 10.1006/bbrc.1996.1206 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1996 Aug 14;225(2):525-30. doi: 
      10.1006/bbrc.1996.1206.