PMID- 8755615
OWN - NLM
STAT- MEDLINE
DCOM- 19961029
LR  - 20240109
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 15
DP  - 1996 Jul 23
TI  - Action spectra for phytochrome A- and B-specific photoinduction of seed 
      germination in Arabidopsis thaliana.
PG  - 8129-33
AB  - We have examined the seed germination in Arabidopsis thaliana of wild type (wt), 
      and phytochrome A (PhyA)- and B (PhyB)-mutants in terms of incubation time and 
      environmental light effects. Seed germination of the wt and PhyA-null mutant 
      (phyA) was photoreversibly regulated by red and far-red lights of 10-1,000 
      micromol m-2 when incubated in darkness for 1-14 hr, but no germination occurred 
      in PhyB-null mutant (phyB). When wt seeds and the phyB mutant seeds were 
      incubated in darkness for 48 hr, they synthesized PhyA during dark incubation and 
      germinated upon exposure to red light of 1-100 nmol m-2 and far-red light of 
      0.5-10 micromol m-2, whereas the phyA mutant showed no such response. The results 
      indicate that the seed germination is regulated by PhyA and PhyB but not by other 
      phytochromes, and the effects of PhyA and PhyB are separable in this assay. We 
      determined action spectra separately for PhyA- and PhyB-specific induction of 
      seed germination at Okazaki large spectrograph. Action spectra for the PhyA 
      response show that monochromatic 300-780 nm lights of very low fluence induced 
      the germination, and this induction was not photoreversible in the range 
      examined. Action spectra for the PhyB response show that germination was 
      photoreversibly regulated by alternate irradiations with light of 0.01-1 mmol m-2 
      at wavelengths of 540-690 nm and 695-780 nm. The present work clearly 
      demonstrated that PhyA photoirreversibly triggers the germination upon 
      irradiations with ultraviolet, visible and far-red light of very low fluence, 
      while PhyB controls the photoreversible effects of low fluence.
FAU - Shinomura, T
AU  - Shinomura T
AD  - Advanced Research Laboratory, Hitachi Ltd., Saitama, Japan.
FAU - Nagatani, A
AU  - Nagatani A
FAU - Hanzawa, H
AU  - Hanzawa H
FAU - Kubota, M
AU  - Kubota M
FAU - Watanabe, M
AU  - Watanabe M
FAU - Furuya, M
AU  - Furuya M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (PHYA protein, Arabidopsis)
RN  - 0 (PHYB protein, Arabidopsis)
RN  - 0 (Phytochrome A)
RN  - 0 (Transcription Factors)
RN  - 11121-56-5 (Phytochrome)
RN  - 136250-22-1 (Phytochrome B)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Arabidopsis/*physiology/radiation effects
MH  - Arabidopsis Proteins
MH  - Darkness
MH  - Immunohistochemistry
MH  - Kinetics
MH  - Light
MH  - Mutation
MH  - Photons
MH  - Photoreceptor Cells
MH  - Phytochrome/biosynthesis/chemistry/*metabolism
MH  - Phytochrome A
MH  - Phytochrome B
MH  - Seeds/*physiology/radiation effects
MH  - *Transcription Factors
PMC - PMC38887
EDAT- 1996/07/23 00:00
MHDA- 1996/07/23 00:01
PMCR- 1997/01/23
CRDT- 1996/07/23 00:00
PHST- 1996/07/23 00:00 [pubmed]
PHST- 1996/07/23 00:01 [medline]
PHST- 1996/07/23 00:00 [entrez]
PHST- 1997/01/23 00:00 [pmc-release]
AID - 10.1073/pnas.93.15.8129 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8129-33. doi: 
      10.1073/pnas.93.15.8129.