PMID- 8760062
OWN - NLM
STAT- MEDLINE
DCOM- 19961203
LR  - 20191210
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 271
IP  - 1 Pt 1
DP  - 1996 Jul
TI  - Ca(2+)-dependent nitric oxide release in endothelial but not R3230Ac rat mammary 
      adenocarcinoma cells.
PG  - C332-7
AB  - We have characterized the ability of several cell types associated with the 
      microvasculature of solid tumors to release nitric oxide (NO.) in response to 
      increases in cytosolic Ca2+ concentration ([Ca2+]c). EA.hy926 immortalized human 
      umbilical vein endothelial cells (EC), rat fibroblasts (RFL), and tumorigenic 
      cells isolated from R3230Ac rat mammary adenocarcinoma (MaC) were treated with 
      thapsigargin (TG), an inhibitor of Ca(2+)-ATPase. NO. output was measured via a 
      chemiluminescence detection system. Baseline NO. output was detectable only for 
      EC. TG caused a significant increase in EC NO. output that could be blocked with 
      NG-monomethyl-L-arginine and restored with L-arginine. TG did not stimulate NO. 
      release from RFL or MaC cells, despite elevating [Ca2+]c in all cells. A 
      Ca(2+)-dependent isoform of NO synthase (eNOS) was detected by immunoblot only in 
      EC. These data indicate that EC, but not RFL or MaC, are capable of 
      Ca(2+)-dependent NO. release and suggest that any Ca(2+)-dependent NO. release 
      within this tumor is primarily of endothelial (and not tumorigenic cell) origin.
FAU - Lindberg, R A
AU  - Lindberg RA
AD  - Department of Radiation Oncology, Duke University Medical Center, Durham 27710, 
      USA.
FAU - Dewhirst, M W
AU  - Dewhirst MW
FAU - Buckley, B J
AU  - Buckley BJ
FAU - Hughes, C S
AU  - Hughes CS
FAU - Whorton, A R
AU  - Whorton AR
LA  - eng
GR  - CA-40355/CA/NCI NIH HHS/United States
GR  - HL-44740/HL/NHLBI NIH HHS/United States
GR  - HL-51183/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Isoenzymes)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 67526-95-8 (Thapsigargin)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenocarcinoma/*metabolism/pathology
MH  - Animals
MH  - Arginine/pharmacology
MH  - Calcium/*physiology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Cell Line
MH  - Endothelium, Vascular/*metabolism
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Immunoblotting
MH  - Isoenzymes/metabolism
MH  - Mammary Neoplasms, Experimental/*metabolism/pathology
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase/antagonists & inhibitors/metabolism
MH  - Rats
MH  - Thapsigargin/pharmacology
MH  - omega-N-Methylarginine/pharmacology
EDAT- 1996/07/01 00:00
MHDA- 1996/07/01 00:01
CRDT- 1996/07/01 00:00
PHST- 1996/07/01 00:00 [pubmed]
PHST- 1996/07/01 00:01 [medline]
PHST- 1996/07/01 00:00 [entrez]
AID - 10.1152/ajpcell.1996.271.1.C332 [doi]
PST - ppublish
SO  - Am J Physiol. 1996 Jul;271(1 Pt 1):C332-7. doi: 10.1152/ajpcell.1996.271.1.C332.